Calcein Orange Diacetate is a fluorogenic dye that is used to assess cell viability. It is hydrolyzed to the fluorescent probe Calcein Orange . Calcein Orange is retained by living cells and displays excitation/emission maxima of 525/550 nm, respectively. Calcein Orange Diacetate is an orange-emitting variant of the green fluorophore Calcein AM , which has been used to evaluate cell viability and cytotoxicity.
3,6-diacetoxy Phthalonitrile is a fluorescent probe for monitoring intracellular pH by flow cytometry in living cells. It rapidly crosses cell membranes and is cleaved by cytosolic esterases to form the fluorescent pH indicator 2,3-dicyano-hydroquinone (DCH), which is excited at UV wavelengths. DCH can be excited at 351 nm, with pH-dependent emission in the range of 450-476 nm (pH 5.0-10.0) using a potassium-based buffer.
7-Fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F) is a thiol-reactive fluorogenic probe.1It has been used to quantify the levels of homocysteine, cysteine, and cysteamine in human plasma.2SBD-F displays excitation/emission maxima of 380/515 nm, respectively.
5(6)-Carboxy-2’,7’-dichlorofluorescein diacetate is an oxidant-sensitive and cell-permeable fluorescent probe.1Base hydrolysis of the ester bonds by intracellular esterases releases the acetate groups and the resulting compound, 5(6)-carboxy-2’,7’,-dichlorofluorescein, is oxidized by reactive oxygen species (ROS) and nitric oxide (NO) and displays excitation/emission maxima of 504 and 530 nm, respectively, which can be quantified as a measure of oxidant production.
1.Hempel, S.L., Buettner, G.R., O’Malley, Y.Q., et al.Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: Comparison with 2’,7’-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123Free Radic. Biol. Med.27(1-2)146-159(1999)