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Anti-alpha Internexin Antibody (3U304) 是一种 Rabbit 抗体,靶向 alpha Internexin。Anti-alpha Internexin Antibody (3U304) 可用于 WB,ICC/IF,IHC,FCM,IP。
Anti-alpha Internexin Antibody (3U304) 是一种 Rabbit 抗体,靶向 alpha Internexin。Anti-alpha Internexin Antibody (3U304) 可用于 WB,ICC/IF,IHC,FCM,IP。
规格 | 价格 | 库存 | 数量 |
---|---|---|---|
50 μL | ¥ 1,485 | 5日内发货 | |
100 μL | ¥ 2,490 | 5日内发货 |
产品描述 | Anti-alpha Internexin Antibody (3U304) is a Rabbit antibody targeting alpha Internexin. Anti-alpha Internexin Antibody (3U304) can be used in WB,ICC/IF,IHC,FCM,IP. |
别名 | αInternexin, α Internexin, TXBP1, TXBP 1, NF-66, NF66, NF 66, Neurofilament-66, Neurofilament 66 tax binding protein, Neurofilament 66, Neurofilament 5 (66kD), Neurofilament 5, NEF5, NEF 5, MGC12702, Internexin neuronal intermediate filament protein alpha, INA, Alpha-Inx, Alpha-internexin, alphaInternexin, Alpha Inx, AINX_HUMAN, aInternexin, a Internexin, 66 kDa neurofilament protein |
Ig Type | IgG |
交叉反应 | Human,Mouse,Rat |
验证活性 | 1. Western blot analysis of alpha Internexin on different lysates using anti-alpha Internexin antibody at 1/1,000 dilution. Positive control:Lane 1: Mouse brain tissue, Lane 2: A549. 2. Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-alpha Internexin antibody. Counter stained with hematoxylin. 4. ICC staining alpha Internexin in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. Flow cytometric analysis of M2A cells with alpha Internexin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
应用 | |
推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100 |
抗体种类 | Monoclonal |
宿主来源 | Rabbit |
构建方式 | Recombinant Antibody |
纯化方式 | ProA affinity purified |
性状 | Liquid |
缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
研究背景 | Alpha-internexin is a brain specific type IV intermediate filament protein. This axonal protein is found in most, if not all, neurons of the CNS. The head domain of alpha-internexin is essential for self-assembly into a filament network. Expression levels of alpha-internexin have been shown to be maximal during late embryogenesis and to decline into adulthood, suggesting that this protein plays a role in regulatory processes during the development of the brain. The alpha-internexin promoter has been shown to be activated by Brn-3a or Brn-3c transcription factor binding, while Brn-3b binding to the promoter results in alpha-internexin repression. |
偶联 | Unconjugated |
免疫原 | Recombinant Protein |
Uniprot ID |
分子量 | Theoretical: 66 kDa. |
储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
运输方式 | Shipping with blue ice. |
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