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PVRL1/NECTIN1 Protein, Human, Recombinant (hFc)

产品编号 TMPY-01571

别名: HV1S, ED4, poliovirus receptor-related 1 (herpesvirus entry mediator C), PVRR1, PVRR, SK-12, CD111, PRR, OFC7, PRR1, HIgR, nectin-1, HVEC, CLPED1

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61 kDa and the accession number is Q15223-1.

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PVRL1/NECTIN1 Protein, Human, Recombinant (hFc)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货
1 mg	￥ 24,900		5日内发货

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参考文献

生物活性	Immobilized NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) at 2 μg/ml (100 μl/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag)is 20-120 ng/mL.
产品描述	PVRL1/NECTIN1 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 61 kDa and the accession number is Q15223-1.

种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	Q15223-1
别名	HV1S, ED4, poliovirus receptor-related 1 (herpesvirus entry mediator C), PVRR1, PVRR, SK-12, CD111, PRR, OFC7, PRR1, HIgR, nectin-1, HVEC, CLPED1
蛋白构建	The Human PVRL1 isoform 1 (NP_002846.3) extracellular domain (Met 1-Thr 334) was was fused with the Fc region of Human IgG1 at the C-terminus.
蛋白纯度	> 98 % as determined by SDS-PAGE
分子量	61 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV). CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.

参考文献

1. Yoon M,et al.(2002) Disruption of adherens junctions liberates nectin-1 to serve as receptor for herpes simplex virus and pseudorabies virus entry. J Virol. 76(14): 7203-8. 2. Mata M,et al.(2001) HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system. J Neurovirol. 7(5): 476-80. 3. HAarr L,et al.(2001) Transcription from the gene encoding the herpesvirus entry receptor nectin-1 (HveC) in nervous tissue of adult mouse. Virology. 287(2): 301-9.

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Keywords

PVRL1/NECTIN1 Protein, Human, Recombinant (hFc) CD 111 PVRR 1 ED 4 HV1S herpesvirus entry mediator C ED4 CD-111 poliovirus receptor-related 1 (herpesvirus entry mediator C) PVRR1 CLPED-1 PRR-1 SK 12 poliovirus receptor-related 1 PVRR SK-12 CD111 OFC-7 PRR CLPED 1 OFC 7 OFC7 PVRR-1 PRR1 ED-4 SK12 HIgR nectin-1 HVEC PRR 1 CLPED1 recombinant recombinant-proteins proteins protein

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