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Anti-SMAC Antibody (6G724)
还可以产品编号 TMAY-00649
Anti-SMAC Antibody (6G724) 是一种 Mouse 抗体，靶向 SMAC。Anti-SMAC Antibody (6G724) 可用于 ICC/IF。
Anti-SMAC Antibody (6G724)
Anti-SMAC Antibody (6G724)
还可以产品编号 TMAY-00649
Anti-SMAC Antibody (6G724) 是一种 Mouse 抗体，靶向 SMAC。Anti-SMAC Antibody (6G724) 可用于 ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-SMAC Antibody (6G724) is a Mouse antibody targeting SMAC. Anti-SMAC Antibody (6G724) can be used in ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 6G724 |
| 交叉反应 | Human |
| 验证活性 | Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human Diablo monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm. |
| 应用 | ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SMAC / Diablo (rh SMAC / Diablo ; TMPY-00924; NP_063940.1; Ala56-Asp239). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Human SMAC / Diablo protein (TMPY-00924) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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