购物车
  • 全部删除
  • TargetMol
    您的购物车当前为空

Anti-TGF beta 1/2/3 Polyclonal Antibody

Rating icon 还可以
Anti-TGF beta 1/2/3 Polyclonal Antibody
产品编号 TMAB-01828

Anti-TGF beta 1/2/3 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TGF beta 1/2/3。Anti-TGF beta 1/2/3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

Anti-TGF beta 1/2/3 Polyclonal Antibody

Anti-TGF beta 1/2/3 Polyclonal Antibody

Rating icon 还可以
Anti-TGF beta 1/2/3 Polyclonal Antibody
产品编号 TMAB-01828

Anti-TGF beta 1/2/3 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 TGF beta 1/2/3。Anti-TGF beta 1/2/3 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF,FCM。

规格价格库存数量
50 μL
¥ 1,160
5日内发货
100 μL
¥ 1,970
5日内发货
200 μL
¥ 2,780
5日内发货
大包装 & 定制
加入购物车
TargetMol 的所有产品仅用作科学研究或药证申报,不能被用于人体,我们不向个人提供产品和服务。请您遵守承诺用途,不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多
联系我们获取更多批次信息
资源下载

产品介绍

生物活性
产品描述
Anti-TGF beta 1/2/3 Polyclonal Antibody is a Rabbit antibody targeting TGF beta 1/2/3. Anti-TGF beta 1/2/3 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,FCM.
Ig Type
IgG
交叉反应
Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Sheep,GuineaPig)
验证活性
1. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated (TMAB-01828) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample: Spleen (Rabbit) Lysate at 40 μg
Primary: Anti-TGF Beta 1+2+3 (TMAB-01828) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (secondary antibody) at 1/5000 dilution
Predicted band size: 12.8/44 kDa
Observed band size: 44 kDa
3. Sample: Stomach (mouse) Lysate at 40 μg
Primary: Anti-TGF Beta 1+2+3 (TMAB-01828) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12.8/44 kDa
Observed band size: 44 kDa
4. Blank control: K562. Primary Antibody (green line): Rabbit Anti-TGF Beta 1+2+3 antibody (TMAB-01828)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1%PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 2μg/Test
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted, extracellular space, extracellular matrix.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
抗原信息
免疫原
KLH conjugated synthetic peptide: human TGF Beta 1
抗原种属
Human
基因ID
Uniprot ID
研究领域
Response to hypoxia,TGF,Angiogenic growth factors,TGF,Co-factors,Hypoxia,tgf beta 1,TGF,Secreted
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

  • 稀释 计算器

评论列表

4个月前
5.0
Rating icon 很棒

评论内容

Related Tags: buy Anti-TGF beta 1/2/3 Polyclonal Antibody | purchase Anti-TGF beta 1/2/3 Polyclonal Antibody | Anti-TGF beta 1/2/3 Polyclonal Antibody cost | order Anti-TGF beta 1/2/3 Polyclonal Antibody