DMOG (Dimethyloxalylglycine) is an antagonist of α-ketoglutarate cofactor, an inhibitor of HIF prolyl hydroxylase (HIF-PH), which leads to the accumulation and stabilization of the protein HIF-1α. DMOG induces autophagy, and is a pro-angiogenic agent.

Hydroxyproline in chicken embryo lungs (medium):3.7 μM, Cellular permeable prolyl-4-hydroxylase:5.18 μM, Hydroxyproline (chicken embryo lungs):9.3 μM, JMJD2A:2.5 μM

To analyze DNA synthesis as an index of cellular proliferation, VSMC are plated in 48-well plates (5,000 per square centimeter) in growth medium, incubated overnight, and serum-deprived (1% FCS) for 24 h. Replicate wells are then stored at −70°C for baseline (day 0) cell counts, and fresh medium with or without growth factors is added to the remaining wells, which are incubated 72-96 h in 20 or 5% O2. Days 0 and 3 or 4 cell counts are determined by lysing cells in a buffer containing a fluorescent dye, which has minimal fluorescence by itself but fluoresces when bound to DNA or RNA. Absolute cell numbers are calculated by comparing the fluorescence of specimens with that of a standard curve similarly prepared using a known number of cells. (Only for Reference)

H2O: 17.54 mg/mL (100.15 mM), Sonication is recommended. The compound is unstable in aqueous solution. Please use soon.

DMSO: 60 mg/mL (342.58 mM), Sonication is recommended. The compound is unstable in solution, please use soon.

Ethanol: 35 mg/mL (199.84 mM), Sonication is recommended.

PBS: 30 mg/mL (171.29 mM), Sonication is recommended.