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顶部生物活性储存和溶解信息计算器

Anti-SMAC Antibody (2W730)

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Anti-SMAC Antibody (2W730)
产品编号 TMAC-01147
别名 SMAC, diablo, IAP-binding mitochondrial protein, DFNA64

Anti-SMAC Antibody (2W730) 是一种 Rabbit 抗体，靶向 SMAC。Anti-SMAC Antibody (2W730) 可用于 WB,ICC/IF,IHC,IP,FCM。

Anti-SMAC Antibody (2W730)

Anti-SMAC Antibody (2W730)

Rating icon 还可以
Anti-SMAC Antibody (2W730)
产品编号 TMAC-01147 别名 SMAC, diablo, IAP-binding mitochondrial protein, DFNA64

Anti-SMAC Antibody (2W730) 是一种 Rabbit 抗体，靶向 SMAC。Anti-SMAC Antibody (2W730) 可用于 WB,ICC/IF,IHC,IP,FCM。

规格价格库存数量
50 μL
¥ 1,480
5日内发货
100 μL
¥ 2,495
5日内发货
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产品介绍

生物活性
产品描述
Anti-SMAC Antibody (2W730) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (2W730) can be used in WB,ICC/IF,IHC,IP,FCM.
别名SMAC, diablo, IAP-binding mitochondrial protein, DFNA64
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Western blot analysis of DIABLO on different lysates using anti-DIABLO antibody at 1/1,000 dilution. Positive control: Lane 1: Jurkat, Lane 2: Hela, Lane 3: MCF-7.
2. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
3. Immunohistochemical analysis of paraffin-embedded mouse overy tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
4. Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DIABLO antibody. Counter stained with hematoxylin.
5. ICC staining DIABLO in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
6. ICC staining DIABLO in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
7. ICC staining DIABLO in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
8. ICC staining DIABLO in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
9. Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
应用
WB
ICC/IF
IHC
IP
FCM
推荐剂量
WB: 1:1000-2000; IHC: 1:50-200; ICC/IF: 1:50-200; FCM: 1:50-100
抗体种类
Monoclonal
宿主来源Rabbit
构建方式Recombinant Antibody
纯化方式ProA affinity purified
性状Liquid
缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
研究背景The activation of caspases is a key regulatory step in apoptosis. Once cytochrome c is released from the mitochondria into the cytosol, it binds Apaf-1 to form an oligomeric cytochrome c/Apaf-1 complex, which induces caspase activation. Inhibitors of Apoptosis Proteins (IAPs), are a family of proteins that regulate the cytochrome c/Apaf-1 caspase activating pathway. Like cytochrome c, Smac (for second mitochondria-derived activator of caspase, also designated DIABLO in mouse for direct IAP binding protein with low PI) promotes caspase activation in the cytochrome c/Apaf-1/caspase-9 pathway by binding IAPs and preventing them from inhibiting caspases. In healthy cells, Smac is a mitochondrial protein, but when cells undergo apoptosis, Smac is released into the cytosol.
偶联与修饰
偶联
Unconjugated
抗原信息
免疫原
Recombinant Protein
Uniprot ID
Q9NR28
化学信息
分子量Theoretical: 21 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

计算器

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关键词

DFNA-64DFNA 64

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