Anti-SMAC Antibody (1X374) is a Rabbit antibody targeting SMAC. Anti-SMAC Antibody (1X374) can be used in WB,ELISA,FCM,ICC/IF.

Monoclonal Rabbit IgG

1X374

Human

1. Anti-Diablo rabbit monoclonal antibody at 1:10000 dilution.

-Lane A: Hela Whole Cell Lysate.

-Lane B: Jurkat Whole Cell Lysate.

-Lane C: A431 Whole Cell Lysate.

-Lane D: MCF7 Whole Cell lysate.

-Lysates/proteins at 30 μg per lane.

-Secondary

-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

-Developed using the Odyssey technique.

-Performed under reducing conditions.

-Predicted band size:22 kDa.

-Observed band size:20 kDa.

2. Immunofluorescence staining of Human Diablo in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Diablo monoclonal antibody (1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

3. Flow cytometric analysis of Human Diablo expression on HeLa cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human Diablo (Filled hisgram), then a FITC-conjugated second step antibody. To demonstrate specificity of staining, the binding by Anti-SMAC Antibody was blocked by preincubation of the purified antibody with 20ug recombinant human Diablo for 1 hour (Black solid line hisgram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

WB,ELISA,FCM,ICC/IF

WB: 1:10000-1:50000; ELISA: 1:5000-1:10000; ICC-IF: 1:20-1:100; FCM: 1:25-1:100

Monoclonal

Unconjugated

Recombinant Human SMAC / Diablo protein (TMPY-00924)

Human