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Anti-DGAT1 Polyclonal Antibody
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产品编号 TMAB-00542
Anti-DGAT1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DGAT1。Anti-DGAT1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-DGAT1 Polyclonal Antibody
Anti-DGAT1 Polyclonal Antibody
还可以产品编号 TMAB-00542
Anti-DGAT1 Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DGAT1。Anti-DGAT1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,785 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-DGAT1 Polyclonal Antibody is a Rabbit antibody targeting DGAT1. Anti-DGAT1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse (predicted:Rat,Dog,Pig,Horse) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DGAT1) Polyclonal Antibody, Unconjugated (TMAB-00542) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: Lane 1: Mouse Adipose tissue lysates Lane 2: Mouse Adrenal gland tissue lysates Lane 3: Rat Adipose tissue lysates Lane 4: Rat Stomach tissue lysates Lane 5: Rat Testis tissue lysates Lane 6: Human HL60 cell lysates Lane 7: Human HepG2 cell lysates Primary: Anti-DGAT1 (TMAB-00542) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kDa Observed band size: 60 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed. |
| 免疫原 | KLH conjugated synthetic peptide: human DGAT1 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of lipids and lipoproteins,Metabolism,Fatty acids,Lipid metabolism,Fatty acid oxidation,Lipid metabolism,Lipid Signaling |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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