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Anti-EpCAM/TROP1 Antibody (9H261)
还可以产品编号 TMAY-01076
Anti-EpCAM/TROP1 Antibody (9H261) 是一种 Rabbit 抗体，靶向 EpCAM/TROP1。Anti-EpCAM/TROP1 Antibody (9H261) 可用于 WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP。
Anti-EpCAM/TROP1 Antibody (9H261)
Anti-EpCAM/TROP1 Antibody (9H261)
还可以产品编号 TMAY-01076
Anti-EpCAM/TROP1 Antibody (9H261) 是一种 Rabbit 抗体，靶向 EpCAM/TROP1。Anti-EpCAM/TROP1 Antibody (9H261) 可用于 WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP。
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产品介绍
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偶联与修饰
抗原信息
| 产品描述 | Anti-EpCAM/TROP1 Antibody (9H261) is a Rabbit antibody targeting EpCAM/TROP1. Anti-EpCAM/TROP1 Antibody (9H261) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9H261 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human EpCAM in SKBR3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human EpCAM monoclonal antibody (1:500) at 4°C overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane. 2. Immunochemical staining of human EpCAM in human mammary gland with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of alveolus epithelium. 3. Immunochemical staining of human EpCAM in human colon carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of colonic gland epithelium. 4. Immunochemical staining of human EpCAM in human bladder carcinoma with rabbit monoclonal antibody at 1:2500 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of transitional epithelium. 5. Flow cytometric analysis of anti-EpCAM (CD326) reactivity on SKBR3 cells. SKBR3 cells were stained with anti-EpCAM (CD326) Monoclonal Ab, then a FITC-conjugated second step antibody. The histogram were derived from the gated events based on light scattering characteristics of viable cells. 6. Anti-EpCAM rabbit monoclonal antibody at 1:500 dilution. -Lane A: MCF7 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. -Developed using the Odyssey technique. -Performed under reducing conditions. -Predicted band size:34 kDa. -Observed band size:38 kDa. 7. EpCAM was immunoprecipitated using: -Lane A:0.5 mg MCF-7 Whole Cell Lysate. -Lane B:0.5 mg A431 Whole Cell Lysate. -Lane C:0.5 mg HepG2 Whole Cell Lysate. -Lane D:0.5 mg Caco-2 Whole Cell Lysate -0.5 µL anti-EpCAM rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose. -Primary antibody: -Anti-EpCAM rabbit monoclonal antibody, at 1:5000 dilution. -Secondary antibody: -Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. -Developed using the odyssey technique. -Performed under reducing conditions. -Predicted band size: 40 kDa. -Observed band size: 40 kDa |
| 应用 | WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF,IP |
| 推荐剂量 | WB: 1:500-1:1000; ELISA: 1:25000-1:50000; IHC-P: 1:1000-1:5000; ICC-IF: 1:250-1:5000; FCM: 1:100-1:500; IP: 0.2-1 μL/mg of lysate; ELISA(Det): 1:5000-1:50000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human EpCAM / TROP1 (rh EpCAM; TMPY-01300; NP_002345.1; Met 1-Lys 265). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | Epithelial Cell Adhesion Molecule (EpCAM), also known as GA733-2 antigen, is a type I transmembrane glycoprotein composed of an extracellular domain with two EGF-Like repeats and a cystenin-rich region, a transmembrane domain and a cytoplasmic domain. It modulates cell adhesion and proliferation. Its overexpression has been detected in many epithelial tumours and has been associated with high stage, high grade and a worse survival in some tumour types. EpCAM has been shown to function as a calcium-independent homophilic cell adhesion molecule that does not exhibit any obvious relationship to the four known cell adhesion molecule superfamilies. However, recent insights have revealed that EpCAM participates in not only cell adhesion, but also in proliferation, migration and differentiation of cells. In addition, recent study revealed that EpCAM is the Wnt-beta-catenin signaling target gene and may be used to facilitate prognosis. It has oncogenic potential and is activated by release of its intracellular domain, which can signal into the cell nucleus by engagement of elements of the wnt pathway.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/Biotin/FITC/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human EpCAM protein (TMPY-01300) |
| 抗原种属 | Human |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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