Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CAY10404 (3-(4-METHYLSULPHONYLPHENYL)-4-PHENYL-5-T) 是一种有效且高度选择性的 COX-2 和 COX-1 抑制剂。 它还是 PKB/Akt 和 MAPK 信号通路的有效抑制剂,可诱导 NSC-LC 细胞凋亡,具有镇痛、抗炎和抗癌活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 315 | 现货 | ||
2 mg | ¥ 453 | 现货 | ||
5 mg | ¥ 747 | 现货 | ||
10 mg | ¥ 1,230 | 现货 | ||
25 mg | ¥ 2,660 | 现货 | ||
50 mg | ¥ 4,320 | 现货 | ||
100 mg | ¥ 6,680 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 822 | 现货 |
产品描述 | CAY10404 (3-(4-METHYLSULPHONYLPHENYL)-4-PHENYL-5-T) is a potent and highly selective inhibitor of COX-2 and COX-1. It is also a potent inhibitor of PKB/Akt and MAPK signalling pathways and induces apoptosis in NSC-LC cells, with analgesic, anti-inflammatory and anti-cancer activities. |
靶点活性 | COX-2:1 nM, COX-1:>500 μM |
体外活性 | Treatment with CAY10404 in the range of 10-100 microM caused dose-dependent growth inhibition, with an average 50% inhibitory concentration (IC(50)) of 60-100 micromol/L, depending on the cell line.?Western blot analysis of CAY10404-treated cells showed cleavage of poly (ADP-ribose) polymerase (PARP) and procaspase-3, signifying caspase activity and apoptotic cell death.?CAY10404 treatment inhibited the phosphorylation of Akt, glycogen synthase kinase-3beta and extracellular signal-regulated kinases 1/2 in H460 and H358 cells[1] |
细胞实验 | To measure the effects of CAY10404 on proliferation of NSCLC cells, 3 x 10(3) cells/well were plated in 96-well plates and allowed to adhere overnight at 37 degrees C. After treatment with CAY10404 for 3 days, cell proliferation was measured by the 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In the H460 NSCLC cells, evidence of apoptosis was sought using the terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labelling (TUNEL) assay and western blot analysis[1]. |
别名 | 3-(4-METHYLSULPHONYLPHENYL)-4-PHENYL-5-T |
分子量 | 367.34 |
分子式 | C17H12F3NO3S |
CAS No. | 340267-36-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 10 mg/mL (27.22 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.7223 mL | 13.6114 mL | 27.2227 mL | 68.0568 mL |
5 mM | 0.5445 mL | 2.7223 mL | 5.4445 mL | 13.6114 mL | |
10 mM | 0.2722 mL | 1.3611 mL | 2.7223 mL | 6.8057 mL | |
20 mM | 0.1361 mL | 0.6806 mL | 1.3611 mL | 3.4028 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
CAY10404 340267-36-9 Apoptosis Cytoskeletal Signaling Immunology/Inflammation Neuroscience PI3K/Akt/mTOR signaling Akt COX Bcl-XL cancer anti-inflammatory Bcl-2 H-1703 Cyclooxygenase CAY-10404 NSCLC H-358 inhibit cell pGSK-3β H-460 analgesic 3-(4-METHYLSULPHONYLPHENYL)-4-PHENYL-5-T MAPK cyclooxygenase-2 lung anti-cancer Inhibitor Non-small CAY 10404 Protein kinase B pAkt PKB inhibitor