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Anti-COX-2 Antibody (1O260)
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产品编号 TMAB-00471
Anti-COX-2 Antibody (1O260) 是一种 Rabbit 抗体，靶向 COX-2。Anti-COX-2 Antibody (1O260) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-COX-2 Antibody (1O260)
Anti-COX-2 Antibody (1O260)
还可以产品编号 TMAB-00471
Anti-COX-2 Antibody (1O260) 是一种 Rabbit 抗体，靶向 COX-2。Anti-COX-2 Antibody (1O260) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,395 | 5日内发货 | |
| 100 μL | ¥ 2,495 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-COX-2 Antibody (1O260) is a Rabbit antibody targeting COX-2. Anti-COX-2 Antibody (1O260) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| Ig Type | IgG |
| 克隆号 | 1O260 |
| 交叉反应 | Human,Mouse |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded Mouse Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 4. Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 6. Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 7. Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 8. Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 9. Paraformaldehyde-fixed, paraffin embedded Human Esophagus Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 10. Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 11. Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 12. Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Cyclooxygenase 2 Monoclonal Antibody, Unconjugated (TMAB-00471) at 1:200 overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining. 13. 25 μg total protein per Lane of various lysates probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (TMAB-00471) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. 14. RAW264.7 (M) cells were treated with or without LPS+BFA (100 ng/mL) for 6 h, 25 μg total protein per Lane of cell lysates probed with Cyclooxygenase 2 monoclonal antibody, unconjugated (TMAB-00471) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at RT for 60 min. |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:50-200; IF: 1:100-500 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Post-translational modifications : S-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis. [provided by RefSeq, Feb 2009] |
| 免疫原 | A synthesized peptide: human COX2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Hormone biosynthesis,Tumor biomarkers,Lipoprotein metabolism,Platelets,Cholesterol Metabolism,Macrophage / Inflamm.,Hormone biosynthesis,Cholesterol Metabolism,Lipoprotein metabolism,Heart disease,Prostaglandins |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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