规格

价格

库存

数量

1 mg

¥ 113

现货

5 mg

¥ 273

现货

10 mg

¥ 438

现货

25 mg

¥ 745

现货

50 mg

¥ 1,080

现货

1 mL x 10 mM (in DMSO)

¥ 279

现货

生物活性

产品描述	Amifostine thiol dihydrochloride (WR 1065) can activate p53 through a JNK-dependent signaling pathway. It also protects normal tissues from the toxic effects of certain cancer drugs.
体外活性	The DNA-binding activity is increased in a WR-1065 concentration-dependent manner. Cells treated with 1 mM WR-1065 dihydrochloride for 24 h revealed that all of the p53-induced genes analyzed are transactivated following WR-1065 dihydrochloride treatment, in a p53-dependent manner. Significantly, treatment with WR-1065 dihydrochloride leads to a 3-fold increase in luciferase expression driven by AP-1 and a 5-fold increase when this reporter gene is driven by NF-κB when these values are normalized to the level of the cotransfected β-galactosidase gene [2].
体内活性	WR-1065 attenuates the severity of 6-OHDA-induced catalepsy when compared with 6-OHDA-lesioned rats. Also, it has been observed that WR-1065 dihydrochloride improves catalepsy in a dose-dependent manner. Pretreatment with three different doses of WR-1065 dihydrochloride (20, 40, and 80 μg/2 μL/rat) for 3 days before 6-OHDA administration, significantly elevates SOD activity and restores it to normal range compare with 6-OHDA lesioned rats [3].
细胞实验	For Western analysis, cells are treated with 1 mM WR-1065 for 24 h, and subconfluent cultures of cells are harvested and lysed in RIPA buffer supplemented with protease inhibitors. Protein concentrations are determined by a detergent-compatible assay. Western blots are blocked and incubated in antibody in PBS/0.2% Tween 20/5% nonfat dry milk. Blots are incubated with 1 μg/mL antibody for 1 h at room temperature, followed by washing in PBS/0.2% Tween 20 and incubation in peroxidase-conjugated secondary antibody and chemiluminescence detection [2].
动物实验	Seventy-two rats are divided randomly into 9 equal groups: 1) Control group receives no injection and is left untreated for the entire period of the experiment as intact animals; 2) Sham-operated group is subjected only to surgical procedure; 3) Vehicle (saline)-treated group receives 2 μL saline (Intra-SNc); 4) Lesioned group receives 6-hydroxydopamine; 5) Vehicle+6OHDA group receives saline as a vehicle 3 days once daily (2 μL/rat) before 6-OHDA injection; 6 to 8) Rats in these groups are pretreated with Intra-SNc injection of WR-1065 (20, 40 and 80 μg/2 μL/rat) 3 days before 6-OHDA injection; 9) Non-lesioned animals receive intra-SNc injection of WR-1065 dihydrochloride (80 μg/2 μL/rat) for three days [3].
别名	硫代乙基氨基乙基胺盐酸盐, WR 1065

化学信息

分子量	207.165
分子式	C₅H₁₆Cl₂N₂S
CAS No.	14653-77-1
Smiles	Cl.Cl.NCCCNCCS
密度	0.975g/cm3

储存&溶解度

存储

溶解度信息

DMSO: 24 mg/mL (115.85 mM), Sonication is recommended.

H2O: 100 mg/mL (482.71 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (4.83 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO/H2O

	1mg	5mg	10mg	50mg
1 mM	4.8271 mL	24.1354 mL	48.2707 mL	241.3535 mL
5 mM	0.9654 mL	4.8271 mL	9.6541 mL	48.2707 mL
10 mM	0.4827 mL	2.4135 mL	4.8271 mL	24.1354 mL
20 mM	0.2414 mL	1.2068 mL	2.4135 mL	12.0677 mL
50 mM	0.0965 mL	0.4827 mL	0.9654 mL	4.8271 mL
100 mM	0.0483 mL	0.2414 mL	0.4827 mL	2.4135 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Amifostine thiol dihydrochloride

Amifostine thiol dihydrochloride

产品介绍

计算器

体内实验配液计算器

剂量转换

引用文献

关键词

Amifostine thiol dihydrochloride

Amifostine thiol dihydrochloride