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α-Cyano-4-hydroxycinnamic acid

α-Cyano-4-hydroxycinnamic acid

产品编号 T7460   CAS 28166-41-8
别名: α-Cyano-4-hydroxycinnamic acid(α-CHCA), 2-氰基-4-羟基

α-Cyano-4-hydroxycinnamic acid (α-Cyano-4-hydroxycinnamic acid(α-CHCA)) 是非竞争性单羧酸盐转运蛋白 (MCTs) 抑制剂,可抑制线粒体丙酮酸转运蛋白,Ki 值为 6.3 μM。它可作为基质辅助激光解吸电离-飞行时间质谱 (MALDI-TOF) 分析的基质,可以促进肽离子化。

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α-Cyano-4-hydroxycinnamic acid Chemical Structure
α-Cyano-4-hydroxycinnamic acid, CAS 28166-41-8
规格 价格/CNY 货期 数量
50 mg ¥ 313 现货
100 mg ¥ 453 现货
200 mg ¥ 659 现货
1 mL * 10 mM (in DMSO) ¥ 185 现货
药物设计专题培训
千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
产品目录号及名称: α-Cyano-4-hydroxycinnamic acid (T7460)
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纯度: 99.8%
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参考文献
产品描述 α-Cyano-4-hydroxycinnamic acid (α-Cyano-4-hydroxycinnamic acid(α-CHCA)) (α-CHCA) is an inhibitor of monocarboxylate transporter 1 (MCT1) that blocks pyruvate transport in rat heart mitochondria (IC50 : 1.5 μM).
体外活性 The effectiveness of CHC in glioma cells appears to be dependent on Monocarboxylate transporters (MCTs) membrane expression.?MCT1 downregulation showed similar effects on different glioma cells, supporting CHC as an MCT1 inhibitor.?There was a synergistic effect when combining CHC with temozolomide treatment in U251 cells.?In the CAM in vivo model, CHC decreased the size of tumors and the number of blood vessels formed[1].
细胞实验 Cells were plated into 96-well plates, at a density of 3 × 10^3 cells per well.?The effect of treatment with CHC (0.6–12 mM) on cell number (total biomass) was determined at 24, 48, and 72 h by the?sulforhodamine B assay (SRB, TOX-6).?IC50 values (CHC concentration that corresponds to 50% of cell growth inhibition) were estimated from 3 independent experiments, each one in triplicate, using GraphPad Software.?Cell proliferation assay was performed as previously described32 and assessed under the treatment conditions previously described, for 5 mM and 10 mM of CHC.?After CHC treatment, cells were incubated with BrdU and its incorporation was assessed at 450 nm (λref = 655 nm) .?Cell growth (total biomass) and cell proliferation for glioma siMCT1 cells and the effect of CHC (1.25–15 mM) on cell number were performed[1].
别名 α-Cyano-4-hydroxycinnamic acid(α-CHCA), 2-氰基-4-羟基
分子量 189.17
分子式 C10H7NO3
CAS No. 28166-41-8

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 37mg/mL(195.59mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.2863 mL 26.4313 mL 52.8625 mL 132.1563 mL
5 mM 1.0573 mL 5.2863 mL 10.5725 mL 26.4313 mL
10 mM 0.5286 mL 2.6431 mL 5.2863 mL 13.2156 mL
20 mM 0.2643 mL 1.3216 mL 2.6431 mL 6.6078 mL
50 mM 0.1057 mL 0.5286 mL 1.0573 mL 2.6431 mL
100 mM 0.0529 mL 0.2643 mL 0.5286 mL 1.3216 mL

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TargetMol Library Books参考文献

1. Miranda-Goncalves V , Honavar M , Pinheiro C , et al. Monocarboxylate transporters (MCTs) in gliomas: expression and exploitation as therapeutic targets[J]. Neuro-Oncology, 2013, 15(2):172-188. 2. Colen C B , Shen Y , Ghoddoussi F , et al. Metabolic Targeting of Lactate Efflux by Malignant Glioma Inhibits Invasiveness and Induces Necrosis: An In Vivo Study[J]. Neoplasia, 2011, 13(7):620-632.

TargetMol Library Books文献引用

1. Tong Y, Guo C, Liu Z, et al. In situ localization of tris-(2, 3-dibromopropyl) isocyanurate in mouse organs by MALDI-IMS with auxiliary matrix strategy. Talanta. 2021: 122723.
7ACC1 Niflumic acid Lactate transportor 1 AR-C155858 AZD0095 BAY-8002 MCT1-IN-3 7ACC2

相关化合物库

该产品包含在如下化合物库中:
抑制剂库 膜蛋白靶向化合物库 线粒体靶向库 铜死亡化合物库 经典已知活性库 已知活性化合物库 抗癌化合物库 表型筛选靶点鉴定库 NO PAINS 化合物库 共价抑制剂库

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母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

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Keywords

α-Cyano-4-hydroxycinnamic acid 28166-41-8 Membrane transporter/Ion channel Monocarboxylate transporter matrix-assisted MALDI-TOF matrix Inhibitor lactate a-Cyano-4-hydroxycinnamic acid applications MCTs pyruvate laser desorption α-Cyano-4-hydroxycinnamic acid(α-CHCA) spectrometry ionization Monocarboxylate Transporter a-Cyano-4-hydroxycinnamic acid(a-CHCA) transporter α Cyano 4 hydroxycinnamic acid 2-氰基-4-羟基 peptide inhibit alpha-Cyano-4-hydroxycinnamic acid alpha-Cyano-4-hydroxycinnamic acid(alpha-CHCA) αCyano4hydroxycinnamic acid facilitate inhibitor

 

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