规格

价格

库存

数量

1 mg

¥ 179

现货

5 mg

¥ 395

现货

10 mg

¥ 597

现货

25 mg

¥ 1,190

现货

50 mg

¥ 1,790

现货

100 mg

¥ 2,670

现货

1 mL x 10 mM (in DMSO)

¥ 437

现货

生物活性

产品描述	CB1954(Tretazicar (NSC-115829)), an anticancer prodrug, is activated by NAD(P)H quinone oxidoreductase 2. It is converted in the presence of the enzyme NQO2 and co-substrate caricotamide ( EP-0152R) (EP) into a potent cytotoxic bifunctional alkylating agent.
体外活性	在NPC细胞系CNE1中，Tretazicar增强了细胞杀伤效果。在HepG2细胞系中，NOR1基因上调Grb2表达并激活MAPK信号转导，从而增强Tretazicar介导的细胞毒性。
体内活性	NTR/CB1954系统通过剂量依赖的方式，用于体内特异性细胞的消融。NTR介导的CB1954活化产生交联，推测是通过触发凋亡级联反应迅速导致细胞死亡。NTR-CB1954的选择性和强效细胞杀伤作用不需要p53功能。
细胞实验	HepG2 cells,which are maintained in RPMI 1640 supplemented with 10% fetal calf serum (FCS) in a humidified culture incubator at 37?C with 5% CO2 and 95% air, grow to ~80% confluence are washed with PBS and treated with r CB1954(4-10 μmol/L) for 48hours.
动物实验	RED 40 female mice,which express high levels of BLG-NTR transgene in the mammary gland and nontransgenic control mice on lactation day 6, were injected intraperitoneally （i.p.）with 50 mg/kg CB1954 dissolved in arachis oil containing 10% acetone.
别名	NSC 115829, CB1954, 5-Aziridino-2,4-dinitrobenzamide

化学信息

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 47 mg/mL (186.37 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.93 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO