规格

价格

库存

数量

50 mg

¥ 327

现货

100 mg

¥ 460

现货

500 mg

¥ 1,110

现货

1 mL x 10 mM (in DMSO)

¥ 240

现货

生物活性

产品描述	Docetaxel trihydrate (RP-56976 Trihydrate) is an antineoplastic agent that has a unique mechanism of action as an inhibitor of cellular mitosis and that currently plays a central role in the therapy of many solid tumor including breast and lung cancer. Therapy with docetaxel has been associated with a low rate of serum enzyme elevations and rarely to instances of acute hepatic necrosis generally due to severe hypersensitivity reactions or sepsis.
体外活性	每4天注射3次33 mg/kg Docetaxel导致M2OL2结肠癌异种移植瘤的生长延迟19.3天.在MX-1,SK-MEL-2,LX-1和OVCAR-3 异种移植物中,Docetaxel具有良好的抗肿瘤活性.一周注射两次Docetaxel,持续14天,抑制成纤维细胞生长因子2的血管生成反应,IC 50为5.4 mg/kg.当给药10 mg/kg Docetaxel时,会完全抑制小鼠的血管生成.
体内活性	Docetaxel抑制人癌细胞Hs746T (胃),AGS (胃),HeLa (子宫颈),CaSki (子宫颈),BxPC3 (胰腺),Capan-1 (胰腺)的克隆存活,IC50 分别为1 nM,1 nM,0.3 nM,0.3 nM,0.3 nM 和 0.3 nM。DDocetaxel可以抑制两个血管生成因子,胸苷磷酸化酶或VEGF刺激的人脐静脉血管内皮细胞趋药性,IC50 为10 pM。Docetaxel诱导人单核细胞,而不影响RAW 264.7 小鼠巨噬细胞中前列腺素H 合酶-2m (PGHS-2)的表达。
细胞实验	2000 cells in 180 μL of medium are seeded in a 96-well plate, and 20 μL of drug solution is simultaneously added in triplicate to each well. The plate is incubated for 3 days at 37°C in a humidified atmosphere of 5% CO2. On day 3, 25 μL of MTT reagent is added to each well. After 4 h of incubation, the medium is removed by aspiration. 0.2 mL of dimethylsulphoxide (DMSO) is added to each well and thoroughly mixed by using a mechanical plate mixer to dissolve the resulting MTT-formazan crystals. Absorbance at 540 nm (OD) is measured by a reader. (Only for Reference)
别名	多西他赛三水合物, RP-56976 (Trihydrate), RP56976 (NSC 628503) Trihydrate

化学信息

分子量	861.95
分子式	C₄₃H₅₉NO₁₇
CAS No.	148408-66-6
Smiles	O.O.O.CC(=O)O[C@@]12CO[C@@H]1C[C@H](O)[C@]1(C)[C@@H]2[C@H](OC(=O)c2ccccc2)[C@]2(O)C[C@H](OC(=O)C(O)C(NC(=O)OC(C)(C)C)c3ccccc3)C(C)=C([C@@H](O)C1=O)C2(C)C
密度	1.37 g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

Ethanol: 100 mg/mL (116.02 mM), Sonication is recommended.

DMSO: 250 mg/mL (290.04 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (4.64 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1602 mL	5.8008 mL	11.6016 mL	58.0080 mL
5 mM	0.2320 mL	1.1602 mL	2.3203 mL	11.6016 mL
10 mM	0.1160 mL	0.5801 mL	1.1602 mL	5.8008 mL
20 mM	0.0580 mL	0.2900 mL	0.5801 mL	2.9004 mL
50 mM	0.0232 mL	0.1160 mL	0.2320 mL	1.1602 mL
100 mM	0.0116 mL	0.0580 mL	0.1160 mL	0.5801 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Docetaxel trihydrate

Docetaxel trihydrate

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

Docetaxel trihydrate

Docetaxel trihydrate