CLEFMA induces autophagic death in H441 cells[1]. CLEFMA inhibits the viability of H441 and A549 cells, with IC50s of 6.4 and 8.9 μM, respectively[2]. CLEFMA induces apoptosis and reduces the DNA-binding activity of NF-κB in H441 cells in a dose-dependent manner[2].

Daily intraperitoneal injection of 0.2-0.4 mg/kg CLEFMA for 4 weeks, CLEFMA inhibits tumor growth and FDG uptake in tumor tissue in a xenograft mouse model [2]. In addition, CLEFMA downregulates the expression of anti-apoptotic markers cIAP1, Bcl-xL, Bcl-2, and survivin, and induces the cleavage and expression of the pro-apoptotic protein BID [2].