Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Vacquinol-1 能够激活 MAPK 通路,是MKK4特异性激活剂。它可以诱导肝细胞癌细胞凋亡,在多形性胶质母细胞瘤小鼠模型中特异性诱导人胶质母细胞瘤细胞死亡、减缓肿瘤进展并延长生存期。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,300 | 现货 | ||
5 mg | ¥ 2,900 | 现货 | ||
10 mg | ¥ 4,290 | 现货 | ||
25 mg | ¥ 6,850 | 现货 | ||
50 mg | ¥ 9,270 | 现货 | ||
100 mg | ¥ 12,500 | 现货 |
产品描述 | Vacquinol-1 is an MKK4 activator, which rapidly and selectively induces glioma cell death. |
体外活性 | Vacquinol-1 induces rapid and specific cell death of glioma cells with IC50 of 3.14 μM via membrane ruffling, cell rounding, massive macropinocytic vacuole accumulation, ATP depletion, and cytoplasmic membrane rupture. [1] |
体内活性 | In mice bearing U3013 mg tumors, Vacquinol-1 (15 μM intracranial injection, or 20 mg/kg p.o.) attenuates tumor growth and prolonging survival. [1] |
激酶实验 | 1MT enantiomers are solubilized in DMSO containing 0.1N HCl and added at concentrations of 100, 50, and 0 μM to wells containing the reaction mixture with tryptophan (0-200 μM) followed by addition of IDO enzyme. Plates are sealed and incubated 1 hr in a humidified 37°C incubator, after which the reactions are terminated by addition of 12.5 μl 30% TCA per well. Plates are then resealed in plastic wrap, incubated 30 min at 50°C to hydrolyze the reaction product N-formylkynurenine to kynurenine, and centrifuged. Supernatants are transferred to a flat-bottom 96-well plate, mixed with 100 μl Ehrlich reagent and incubated 10 min at room temperature. Absorbance at 490 nm is read[1]. |
细胞实验 | Cell viability is measured using CellTiter-Glo Luminescent Cell Viability Assay according to the manufacturer's instructions.(Only for Reference) |
别名 | Vacquinol 1 |
分子量 | 352.86 |
分子式 | C21H21ClN2O |
CAS No. | 5428-80-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 70 mg/mL (164.4 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.834 mL | 14.1699 mL | 28.3399 mL | 70.8496 mL |
5 mM | 0.5668 mL | 2.834 mL | 5.668 mL | 14.1699 mL | |
10 mM | 0.2834 mL | 1.417 mL | 2.834 mL | 7.085 mL | |
20 mM | 0.1417 mL | 0.7085 mL | 1.417 mL | 3.5425 mL | |
50 mM | 0.0567 mL | 0.2834 mL | 0.5668 mL | 1.417 mL | |
100 mM | 0.0283 mL | 0.1417 mL | 0.2834 mL | 0.7085 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Vacquinol-1 5428-80-8 MAPK JNK NSC 13316 hepatocellular carcinoma NSC-13316 inhibit Inhibitor MKK4-specific activator Vacquinol1 induces apoptosis glioblastoma glioblastoma multiforme Vacquinol 1 HCC activates MAPK pathways NSC13316 inhibitor