Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, T221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, T221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow)

1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAPK8) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Large intestine (Mouse) Lysate at 40 μg
Lane 5: Uterus (Mouse) Lysate at 40 μg
Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) (TMAB-01445) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/54 kDa
Observed band size: 46 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221

Human

MAPK pathway,TLR Signaling,MAPK Pathway