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Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody

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Anti-Phospho-JNK1/2/3 (Thr 183, 183,  221) Polyclonal Antibody
产品编号 TMAB-01445
别名 Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689

Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-JNK1/2/3 (Thr 183, 183, 221)。Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody

Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody

Rating icon 还可以
Anti-Phospho-JNK1/2/3 (Thr 183, 183,  221) Polyclonal Antibody
产品编号 TMAB-01445 别名 Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689

Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody 是一种 Rabbit 抗体,靶向 Phospho-JNK1/2/3 (Thr 183, 183, 221)。Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,170
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100 μL
¥ 1,960
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200 μL
¥ 2,785
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产品描述
Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody is a Rabbit antibody targeting Phospho-JNK1/2/3 (Thr 183, 183, 221). Anti-Phospho-JNK1/2/3 (Thr 183, 183, 221) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
别名Tyrosine protein kinase JAK1, Stress activated protein kinase JNK1, SAPK1, SAPK gamma, SAPK 1, Protein kinase, mitogen-activated, 8, Protein kinase JNK1, PRKM8, PRKM 8, p-JNK1/2/3 (Thr 183, 183, 221), p-JNK1/2/3 (T 183, 183, 221), p54 gamma, MK10, MK09, MK08, Mitogen activated protein kinase 8, MAPK8, MAPK 8, MAP kinase 8, JNK21B1/2, JNK1A2, JNK1/2/3 (p-Thr 183, 183, 221), JNK1/2/3 (p-T 183, 183, 221), JNK 46, JNK 1, JNK, JAK1A, JAK 1A, EC 2.7.11.24, C-JUN kinase 1, c Jun N terminal kinase 1, AI849689
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Dog,Pig,Cow)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MAPK8) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Kidney (Mouse) Lysate at 40 μg
Lane 2: Cerebrum (Mouse) Lysate at 40 μg
Lane 3: Cerebrum (Rat) Lysate at 40 μg
Lane 4: Large intestine (Mouse) Lysate at 40 μg
Lane 5: Uterus (Mouse) Lysate at 40 μg
Lane 6: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221) (TMAB-01445) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46/54 kDa
Observed band size: 46 kDa
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (phospho-JNK1 + JNK2 + JNK3 (T183 + T183 + T221)) Polyclonal Antibody, Unconjugated (TMAB-01445) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Cytoplasm. Nucleus.
构建方式Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.
抗原信息
免疫原
KLH conjugated Synthesised phosphopeptide: human JNK1/JNK2/JNK3 around the phosphorylation site of T183/T183/T221
抗原种属
Human
基因ID
Uniprot ID
研究领域
MAPK pathway,TLR Signaling,MAPK Pathway
化学信息
分子量Theoretical: 42 kDa.
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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