Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Tenovin-6 是 Tenovin-1 的类似物,能抑制二氢乳清酸脱氢酶。它也抑制纯化人 SIRT1、SIRT2 和 SIRT3 蛋白脱乙酰酶活性,IC50分别为 21 μM、10 μM 和 67 μM。它是一种 p53转录活性的激活剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 543 | 现货 | ||
2 mg | ¥ 789 | 现货 | ||
5 mg | ¥ 1,820 | 现货 | ||
10 mg | ¥ 2,930 | 现货 | ||
25 mg | ¥ 4,870 | 现货 | ||
50 mg | ¥ 6,950 | 现货 | ||
100 mg | ¥ 9,390 | 现货 | ||
500 mg | ¥ 18,800 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,930 | 现货 |
产品描述 | Tenovin-6 is a p53 transcriptional activity agonist. |
靶点活性 | SIRT1:21 μM, SIRT3:67 μM, SIRT2:10 μM |
体内活性 | Tenovins are active on mammalian cells at one-digit micromolar concentrations and decrease tumor growth in vivo as single agents. Tenovin-6 (50 mg/kg, i.p.) inhibits the growth of tumor in mice[1]. |
激酶实验 | Assays are carried out using purified components in the Fluor de Lys Fluorescent Assay Systems. Relevant FdL substrates are used at 7 μM and NAD+ at 1 mM. Tenovins are solubilized in DMSO with the final DSMO concentration in the reaction being less than 0.25%. For SirT1 and HDAC8, one unit of enzyme is used per reaction, and for SirT2 and SirT3, five units is used per reaction. Reactions are carried out at 37°C for 1 hr. |
细胞实验 | The MTS assay is used to evaluate cell viability. UM cells are seeded into each well of 96-well plates (5,000 cells/well) and treated the next day with control or Tenovin-6 in an increasing concentrations from 0 to 20?μM for 68?h, and then MTS is added at 20?μL/well to be read at a wave length of 490?nm, the IC50 is determined by curve fitting of the sigmoidal dose-response curve. |
别名 | Tenovin 6 |
分子量 | 454.63 |
分子式 | C25H34N4O2S |
CAS No. | 1011557-82-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 91 mg/mL (200.2 mM)
H2O: 90 mg/mL (198 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 2.1996 mL | 10.998 mL | 21.9959 mL | 54.9898 mL |
5 mM | 0.4399 mL | 2.1996 mL | 4.3992 mL | 10.998 mL | |
10 mM | 0.22 mL | 1.0998 mL | 2.1996 mL | 5.499 mL | |
20 mM | 0.11 mL | 0.5499 mL | 1.0998 mL | 2.7495 mL | |
50 mM | 0.044 mL | 0.22 mL | 0.4399 mL | 1.0998 mL | |
100 mM | 0.022 mL | 0.11 mL | 0.22 mL | 0.5499 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Tenovin-6 1011557-82-6 Autophagy Chromatin/Epigenetic DNA Damage/DNA Repair Metabolism Dehydrogenase Sirtuin Dihydroorotate Dehydrogenase Inhibitor MDM-2/p53 MDM2 Tenovin6 acetylation degradation DHODH Tenovin 6 inhibit protein-deacetylating inhibitor