规格

价格

库存

数量

5 mg

¥ 287

现货

10 mg

¥ 453

现货

25 mg

¥ 822

现货

50 mg

¥ 1,360

现货

100 mg

¥ 2,180

现货

200 mg

¥ 2,990

现货

500 mg

¥ 4,930

现货

1 mL x 10 mM (in DMSO)

¥ 497

现货

生物活性

产品描述	FH535, a Wnt/β-catenin signaling and PPAR inhibitor, exhibits anti-tumor activities.
体内活性	FH535抑制β-连环蛋白/Tcf介导的转录,同时还具有抑制共激活剂GRIP1和β-连环蛋白对PPARδ 和 PPAR的聚集作用。在表达较高的或活跃的Wnt/β-连环蛋白通路癌细胞中,FH535能够抑制细胞增殖。
激酶实验	High-throughput Library Screen: Three copies of the optimized or mutated Tcf-binding element from TOPFLASH or FOPFLASH driving a secreted alkaline phosphatase reporter gene are cloned into pCEP4 plasmid, replacing the cytomegalovirus promoter. The plasmids are transfected into HepG2 cells, and hygromycin-resistant clones are pooled. Library screening is done at 20 μmol/L concentration in HepG2 serum-free media. Hits are tested in the HCT116 cell line for inhibition of TOPFLASH luciferase activity but not for inhibition of a reporter activity controlled from β-actin promoter.
细胞实验	Cell viability is determined by the modified 3H-thymidine incorporation assay. Briefly, cells are plated in 96-well microplates for 24 h and treated in triplicate with various concentrations of the test compound. After 48 h of compound exposure, the cells are incubated for an additional 48 h in compound-free medium. The cells are then incubated in medium containing 3H-thymidine for 24 h, washed and mixed with the scintillant in the 96-well plate. Individual wells are counted with a 96-well scintillation counter and the LC50 is calculated.(Only for Reference)

化学信息

分子量	361.2
分子式	C₁₃H₁₀Cl₂N₂O₄S
CAS No.	108409-83-2
Smiles	Cc1cc(ccc1NS(=O)(=O)c1cc(Cl)ccc1Cl)[N+]([O-])=O
密度	1.566 g/cm3 (Predicted)
颜色	White
物理性状	Solid

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

DMSO: 65 mg/mL (179.96 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.54 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7685 mL	13.8427 mL	27.6855 mL	138.4275 mL
5 mM	0.5537 mL	2.7685 mL	5.5371 mL	27.6855 mL
10 mM	0.2769 mL	1.3843 mL	2.7685 mL	13.8427 mL
20 mM	0.1384 mL	0.6921 mL	1.3843 mL	6.9214 mL
50 mM	0.0554 mL	0.2769 mL	0.5537 mL	2.7685 mL
100 mM	0.0277 mL	0.1384 mL	0.2769 mL	1.3843 mL