Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody is a Rabbit antibody targeting Phospho-PPAR alpha/PPARA alpha (Ser12). Anti-Phospho-PPAR alpha/PPARA alpha (Ser12) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF,ELISA.

IgG

Human,Mouse,Rat,Rabbit (predicted:Dog,Pig,Cow,Horse,GuineaPig)

1. phosphopeptide
non phosphopeptide
2. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-PPAR alpha (Ser12) Polyclonal Antibody, Unconjugated (TMAB-01483) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Liver (Mouse) Lysate at 40 μg
Lane 3: Kidney (Mouse) Lysate at 40 μg
Lane 4: Cerebrum (Mouse) Lysate at 40 μg
Lane 5: Cerebrum (Rat) Lysate at 40 μg
Lane 6: Jurkat (Human) Cell Lysate at 30 μg
Lane 7: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-PPAR alpha (Ser12) (TMAB-01483) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 50 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; ELISA: 1:5000-10000

Polyclonal

KLH conjugated synthesised phosphopeptide: human PPAR alpha around the phosphorylation site of ser12

Human

Metabolism of lipids and lipoproteins,Response to hypoxia,Nuclear hormone receptors,Metabolism,Zinc Finger,Fatty acids,Lipid metabolism,Mitochondrial transcription,Hypoxia,Mitochondrial Biogenesis,Fatty acid oxidation,Obesity