Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Thiocolchicine 是一种有效的微管蛋白聚合抑制剂,IC50 为 2.5 µM,Ki 为 0.7 µM。 Thiocolchicine 诱导细胞凋亡。 Thiocolchicine 可用作 ADC 技术中的 ADC 细胞毒素。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 328 | 现货 | ||
2 mg | ¥ 483 | 现货 | ||
5 mg | ¥ 788 | 现货 | ||
10 mg | ¥ 1,270 | 现货 | ||
25 mg | ¥ 2,830 | 现货 | ||
50 mg | ¥ 4,180 | 现货 | ||
100 mg | ¥ 5,990 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 879 | 现货 |
产品描述 | Thiocolchicine is an effective inhibitor of tubulin polymerization with an IC50 of 2.5 µM and a Ki of 0.7 µM. Thiocolchicine induces cell apoptosis. Thiocolchicine can be used as an ADC cytotoxin in ADC technology. |
靶点活性 | Tubulin polymerization:0.7 µM(Ki), Tubulin polymerization:2.5 µM |
体外活性 | Thiocolchicine (1 nM-100 μM; 24-72 hours) shows a relationship between cell cycle blocking activity and growth inhibition in breast cancer cells. Thiocolchicine inhibits cell proliferation of MDA-MB-231 and multidrug-resistant (MDR) MCF-7 ADRr breast cancer cells with IC50s of 0.6 nM and 400 nM, respectively, as well as MDR CEM-VBL leukemia cells with an IC50 of 50 nM[1]. Thiocolchicine is against MCF-7, LoVo, LoVo/DX, A-549 and BALB/3T3 cells with IC50 values of 0.01 μM, 0.021 μM, 0.398 μM, 0.011 μM and 0.114 μM, respectively[2]. |
分子量 | 415.5 |
分子式 | C22H25NO5S |
CAS No. | 2730-71-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 95 mg/mL (228.64 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4067 mL | 12.0337 mL | 24.0674 mL | 60.1685 mL |
5 mM | 0.4813 mL | 2.4067 mL | 4.8135 mL | 12.0337 mL | |
10 mM | 0.2407 mL | 1.2034 mL | 2.4067 mL | 6.0168 mL | |
20 mM | 0.1203 mL | 0.6017 mL | 1.2034 mL | 3.0084 mL | |
50 mM | 0.0481 mL | 0.2407 mL | 0.4813 mL | 1.2034 mL | |
100 mM | 0.0241 mL | 0.1203 mL | 0.2407 mL | 0.6017 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Thiocolchicine 2730-71-4 Cytoskeletal Signaling Microtubule Associated breast LoVo/DX MDR-positive Microtubule/Tubulin inhibit MCF-7 leukemia Colchicine Apoptosis BALB/3T3 cancer A-549 LoVo Analogue Inhibitor inhibitor