Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC-319726 (ZMC1)是一种突变型 p53R175 再激活剂,可抑制表达 p53R175 突变的成纤维细胞的生长,IC50值为8nM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 293 | 现货 | ||
5 mg | ¥ 483 | 现货 | ||
10 mg | ¥ 785 | 现货 | ||
25 mg | ¥ 1,490 | 现货 | ||
50 mg | ¥ 2,670 | 现货 | ||
100 mg | ¥ 3,980 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 529 | 现货 |
产品描述 | NSC-319726 (ZMC1), a mutant p53R175 reactivator, suppresses growth of fibroblasts that expresses the p53R175 mutation (IC50 = 8 nM). |
靶点活性 | p53 (R175):8 nM |
体外活性 | 在p53(R172H/R172H)小鼠中, NSC319726(5 mg/kg)会产生较强的毒性,存活率低.在TOV112D-p53(R175H) 移植瘤中,NSC319726能够抑制瘤细胞生长. |
体内活性 | 在突变型p53的细胞中,NSC319726(IC50=8 nM)可使突变蛋白发生WT样构象变化,从而修复序列特异性的p53转录。 |
激酶实验 | MTS: 5,000 cells of TOV112D cells (5,000 cells/well, in 100 ml culture) are cultured in 96-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compounds. The growth is measured by MTS reagent and Victor Plate reader instrument after incubation for 3 days. |
细胞实验 | Viability assays are done. The cells (5×104 cells/well, in 1 ml culture) are cultured in a 12-well plate to reach the 50%–60% confluence on the second day when treated with serial dilutions of the compound. The growth is measured by Guava ViaCount reagent and Guava PCA instrument after incubation for 3 days.(Only for Reference) |
分子量 | 234.32 |
分子式 | C11H14N4S |
CAS No. | 71555-25-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 2.3 mg/mL (10 mM)), with gentle warming
DMSO: 23.4 mg/mL (100 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
Ethanol / DMSO | 1 mM | 4.2677 mL | 21.3383 mL | 42.6767 mL | 106.6917 mL |
5 mM | 0.8535 mL | 4.2677 mL | 8.5353 mL | 21.3383 mL | |
10 mM | 0.4268 mL | 2.1338 mL | 4.2677 mL | 10.6692 mL | |
DMSO | 20 mM | 0.2134 mL | 1.0669 mL | 2.1338 mL | 5.3346 mL |
50 mM | 0.0854 mL | 0.4268 mL | 0.8535 mL | 2.1338 mL | |
100 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0669 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ZMC1 71555-25-4 Apoptosis p53 NSC 319726 MDM-2/p53 inhibit ZMC 1 ZMC-1 NSC-319726 NSC319726 Inhibitor inhibitor