Rosmarinic acid (Labiatenic acid) is widely found in plants and has antioxidant, anti-inflammatory, and antibacterial activities. It inhibits MAO-A, MAO-B, and COMT with IC50 of 50.1, 184.6, and 26.7 μM respectively.

IKKβ:12 μM

Rosmarinic acid (RA) is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Human keratinocytes (HaCaT cells) are treated with Rosmarinic acid (0.625, 1.25, 2.5, or 5 μM) and exposed to UVB radiation 1 h later. They are then incubated at 37°C for 48 h. At this time, MTT is added to each well to obtain a total reaction volume of 200 μL. After 4 h incubation, the supernatant is removed by aspiration. The formazan crystals in each well are dissolved in dimethyl sulfoxide (DMSO; 150 μL), and the absorbance at 540 nm is measured on a scanning multi-well spectrophotometer[2].

迷迭香酸, Rosemary acid, Labiatenic acid

H2O: 36.03 mg/mL (100 mM), Sonication is recommended.

DMSO: 72.07 mg/mL (200.02 mM), Sonication is recommended.

5% DMSO+95% Saline: 1.5 mg/mL (4.16 mM), Solution.