规格

价格

库存

数量

5 mg

¥ 119

现货

10 mg

¥ 208

现货

25 mg

¥ 413

现货

50 mg

¥ 672

现货

100 mg

¥ 1,090

现货

1 mL x 10 mM (in DMSO)

¥ 137

现货

生物活性

产品描述	JW 55 (JW55) is an effective and selective β-catenin signaling pathway inhibitor, works by inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).
靶点活性	TNKS2 (auto-PARsylation):830 nM, TNKS1 (auto-PARsylation):1.9 μM
体外活性	JW55是一种针对经典Wnt通路的强效且选择性的抑制剂。在Wnt3a诱导并瞬时转染有ST-Luc（SuperTop-萤火虫素酶）报告基因的HEK293细胞中，JW55展现出以IC50值为470 nM的抑制作用。在SW480细胞中，JW55在1至5μM范围内有效；而在HCT-15细胞中，有效范围扩展至0.01至5μM[1]。
体内活性	JW 55（100 mg/kg，口服）减缓了条件性Apc基因敲除小鼠的肿瘤发展。JW 55减少了Xenopus胚胎中XWnt8诱导的轴向复制和Tamoxifen诱导的条件性APC突变小鼠中的息肉形成[1]。
细胞实验	JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium[1]. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation[1].
别名	JW55

化学信息

分子量	434.48
分子式	C₂₅H₂₆N₂O₅
CAS No.	664993-53-7
Smiles	COc1ccc(cc1)C1(CNC(=O)c2ccc(NC(=O)c3ccco3)cc2)CCOCC1
密度	1.235 g/cm3 (Predicted)

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

Ethanol: 100 mM, Sonication is recommended.

DMSO: 50 mg/mL (115.08 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.6 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

Ethanol/DMSO