JW 55 (JW55) is an effective and selective β-catenin signaling pathway inhibitor, works by inhibition of the PARP domain of tankyrase 1 and tankyrase 2 (TNKS1/2).

TNKS2 (auto-PARsylation):830 nM, TNKS1 (auto-PARsylation):1.9 μM

JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium[1]. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation[1].

JW55

Ethanol: 100 mM, Sonication is recommended.

DMSO: 50 mg/mL (115.08 mM), Sonication is recommended.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (4.6 mM), Sonication is recommended.