Powder: -20°C for 3 years | In solvent: -80°C for 1 year
EW-7195 是一种具有选择性和高效性的 ALK5 (TGFβR1) 抑制剂,IC50 值为 4.83 nM。EW-7195 对 ALK5 的亲和力是 p38α 的 300 多倍。EW-7195 对 TGF-β1 诱导的 Smad 信号转导、上皮间质转化 (EMT) 和乳腺癌向肺转移有抑制作用。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 1,320 | 现货 | ||
5 mg | ¥ 3,320 | 现货 | ||
10 mg | ¥ 5,660 | 现货 | ||
25 mg | ¥ 8,430 | 现货 | ||
50 mg | ¥ 11,300 | 现货 | ||
100 mg | ¥ 15,300 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 3,660 | 现货 |
产品描述 | EW-7195 is a selective and potent inhibitor of ALK5 (TGFβR1) with an IC50 value of 4.83 nM.EW-7195 has an affinity for ALK5 that is more than 300-fold higher than that of p38α.EW-7195 has an inhibitory effect on TGF-β1-induced Smad signaling, epithelial mesenchymal transition (EMT), and breast cancer metastasis to the lungs. |
靶点活性 | ALK5:4.83 nM (IC50), p38α:1.5 μM (IC50) |
体外活性 | In vitro, EW-7195 efficiently inhibits the epithelial-to-mesenchymal transition (EMT), motility, and invasiveness of breast cancer cells. Following a 1.5-hour treatment at concentrations of 0.5-1 µM, EW-7195 effectively blocks TGF-β1-induced phosphorylation of Smad2, subsequently preventing the nuclear translocation of Smad2/3. Additionally, EW-7195 hinders TGF-β1-induced mesenchymal morphology and transcriptional activation[1]. |
体内活性 | In both 4T1 orthotopic xenograft and MMTV/cNeu transgenic mice, EW7195 (40 mg/kg; intraperitoneal; three times a week for 3/2.5 weeks) inhibits the development of lung metastasis[1]. |
分子量 | 406.44 |
分子式 | C23H18N8 |
CAS No. | 1352609-28-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40 mg/mL (98.42 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.4604 mL | 12.3019 mL | 24.6039 mL | 61.5097 mL |
5 mM | 0.4921 mL | 2.4604 mL | 4.9208 mL | 12.3019 mL | |
10 mM | 0.246 mL | 1.2302 mL | 2.4604 mL | 6.151 mL | |
20 mM | 0.123 mL | 0.6151 mL | 1.2302 mL | 3.0755 mL | |
50 mM | 0.0492 mL | 0.246 mL | 0.4921 mL | 1.2302 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
EW-7195 1352609-28-9 Angiogenesis Stem Cells Tyrosine Kinase/Adaptors TGF-beta/Smad ALK EW 7195 EW7195 Inhibitor inhibitor inhibit