AAPK-25, a potent and selective dual inhibitor of Aurora/PLK, causes mitotic delay and cell arrest in prometaphase, via phosphorylation of the biomarker histone H3Ser10, followed by a surge in apoptosis. AAPK-25 targets Aurora A, Aurora B, and Aurora C with Kd values ​​ranging from 23-289 nM, and PLK1, PLK2, and PLK3 with Kd values ​​ranging from 55-456 nM. AAPK-25 has antitumor activity.

ERK:5.32 μM (kd), Aurora C:289 nM (kd), PI3K:7.11 μM (kd), CDK:8.02 μM , PLK2:272 nM (kd), PLK1:55 nM (kd), Aurora B:78 nM (kd), Aurora A:23 nM (kd), PLK3:456 nM (kd)