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PLK1 Protein, Human, Recombinant (His)

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产品编号 TMPY-04376

PLK1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 70.5 kDa and the accession number is P53350.

PLK1 Protein, Human, Recombinant (His)

一键复制产品信息

产品编号 TMPY-04376

PLK1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 70.5 kDa and the accession number is P53350.

规格	价格	库存
5 μg	¥ 585	6-8日内发货
10 μg	¥ 958	6-8日内发货
20 μg	¥ 1,590	5日内发货
50 μg	¥ 3,170	In stock
100 μg	¥ 5,420	5日内发货
200 μg	¥ 9,260	5日内发货
500 μg	¥ 18,700	5日内发货

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产品信息

生物活性	The specific activity was determined to be ＞3 nmol/min/mg using casein as substrate.
产品描述	PLK1 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 70.5 kDa and the accession number is P53350.
种属	Human
表达系统	Baculovirus Insect Cells
标签	N-His
蛋白编号	P53350
别名	STPK13,polo-like kinase 1,PLK
蛋白构建	A DNA sequence encoding the human PLK1 (NP_005021.2) (Met 1-Ser 603) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: His
蛋白纯度	≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	70.5 kDa (predicted); 66 kDa (reducing conditions)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
蛋白性状	Solution
缓冲液	Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 0.5 mM PMSF, 25% glycerol.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
研究背景	Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well-characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.