Anti-Aurora B Antibody (9I737) is a Rabbit antibody targeting Aurora B. Anti-Aurora B Antibody (9I737) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

9I737

Human,Mouse

1. Sample:
Lane 1: Human Hela cell Lysates
Lane 2: Human 293T cell Lysates
Primary: Anti-Aurora B (TMAB-00174) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 35 kDa
2. Blocking buffer: 5% NFDM/TBST
Primary dilution: 1:1000
Primary incubation condition: 2 hours at room temperature
Secondary: Goat Anti-Rabbit IgG H&L (HRP)
Lysate: 1: HeLa, 2: 293, 3: A20
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 39 kDa
Observed MW: 39 kDa
3. Cell line: A549
Fixative: 100% Ice-cold methanol
Permeabilization: 0.1% TritonX-100
Primary dilution: 1:50
Primary incubation condition: 4°C overnight
Secondary: Goat Anti-Rabbit IgG
Nuclear counter stain: DAPI (Blue)
Comment: Color green is the positive signal for TMAB-00174
4. Tissue: Human tonsil
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00174
5. Tissue: Mouse spleen
Section type: Formalin fixed & Paraffin-embedded section
Retrieval method: High temperature and high pressure
Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary dilution: 1:100
Primary incubation condition: 1 hour at room temperature
Secondary: SP Kit (Rabbit)
Counter stain: Hematoxylin (Blue)
Comment: Color brown is the positive signal for TMAB-00174

WB: 1:200-1000; IHC-P: 1:20-100; IHC-Fr: 1:400-800; ICC/IF: 1:20-50; IF: 1:20-50

Monoclonal

KLH conjugated synthetic peptide: human Aurora B

Human

Aurora,Aurora,Phosphorylation,Aurora