Powder: -20°C for 3 years | In solvent: -80°C for 2 years
ML324 是 jumonji 组蛋白去甲基化酶的特异性抑制剂,IC50值为920 nM。
产品描述 | ML324(IC50=920 nM) is a specific inhibitor of jumonji histone demethylase (JMJD2). |
靶点活性 | JMJD2:920 nM |
体外活性 | ML324 exhibits good Caco-2 cell permeability, and possesses excellent microsomal stability in the presence of both mouse and rat liver microsomes. ML324 demonstrates potent anti-viral activity against both herpes simplex virus (HSV) and human cytomegalovirus (hCMV) infection by inhibiting viral IE gene expression. [1] |
体内活性 | In a mouse ganglia explant model of latently infected mice, ML324 suppresses the formation of HSV plaques, and blocks HSV-1 reactivation. [1] |
激酶实验 | The fluorescence resonance energy transfer (FRET)-based LANCE Ultra KinaSelect Ser/Thr kit is used to determine IC50 values for various CDK inhibitors. Briefly, a specific ULight MBP peptide substrate (50 nM final concentration) is phosphorylated by a CDK-cyclin pair in buffer (50 mM HEPES-KOH pH 7.5, 10 mM MgCl2, 1 mM EGTA, 2 mM dithiothreitol) containing ATP at the concentration of the Km values of the individual kinases for 1 h at room temperature. Subsequently, phosphorylation is detected by addition of specific Eu-labelled anti-phospho-antibodies (2 nM), which upon binding to the phosphopeptide give rise to a FRET signal. FRET signals are then recorded[1]. |
分子量 | 349.43 |
分子式 | C21H23N3O2 |
CAS No. | 1222800-79-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Ethanol: 3 mg/mL (8.58 mM), warmed
DMSO: 40 mg/mL (114.5 mM)
H2O: <1 mg/mL
( < 1 mg/mL refers to the product slightly soluble or insoluble )
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ML324 1222800-79-4 Chromatin/Epigenetic Microbiology/Virology Others HSV Histone Demethylase ML-324 Jumonji periodontal Cytomegalovirus inhibit simplex CMV Inhibitor Herpes simplex virus human disease H3K9 ML 324 herpes virus domain immunity periodontitis inflammation containing proteins inhibitor