inS3-54A18 inhibits the expression of STAT3 downstream target genes and the binding of STAT3 to its endogenous target sequences. inS3-54A18 (5 μM) decreases wound healing to 64% and 76% for A549 and MDA-MB-231 cells, respectively. inS3-54A18 at 10 μM further reduces the healing to 47% and 39%, respectively. inS3-54A18 does not affect the constitutive or IL-6-induced STAT3 activation but represses the STAT3 target gene (survivin) expression[1].

inS3-54A18 (200 mg/kg, p.o.) inhibits tumor growth, metastasis. In a mouse xenograft model of A549 cells, inS3-54A18 (200 mg/kg, p.o.) also inhibits expression of STAT3 target genes[1].