Powder: -20°C for 3 years | In solvent: -80°C for 2 years
Abemaciclib methanesulfonate 是一种选择性的CDK4/6抑制剂,能够抑制 CDK4/CDK6 的活性,IC50分别为 2 nM 和 10 nM。
产品描述 | LY2835219 is a specific and effective inhibitor of CDK4(IC50=2 nM) and CDK6(IC50=10 nM). |
靶点活性 | CDK4:2 nM, CDK6:10 nM |
体外活性 | MsOH处理皮下和颅内胶质瘤模型(U87 mg)中,LY2835219以剂量依赖的方式抑制肿瘤生长. |
体内活性 | 在视网膜母细胞瘤中,LY2835219通过特异性抑制CDK4和6,使细胞周期停滞在G1期,抑制DNA合成,且抑制癌细胞生长。 |
激酶实验 | Cells (5 × 103) are plated in 96 well plates. Cells are treated the next day for 24 to 48 hours and then assessed for caspase-3 activity by Caspase-Glo-3/7 Assay, as per manufacturer's instructions and a luminescence plate reader. |
细胞实验 | LY2835219 is dissolved in DMSO to a 10 mM concentration.? Cells are seeded in a 96-well plate, allowed to adhere overnight, and treated with DMSO control (0.1% v/v) or the indicated compounds for 72 h. Cell viability and proliferation are determined using a Cell Counting Kit according to the manufacturer's instructions. The interaction between LY2835219 and mTOR inhibitor is determined using CompuSyn. Combination index (CI) values of 1 indicates and additive drug interaction, whereas a CI of < 1 is synergistic and a CI of > 1 is antagonistic. |
别名 | abemaciclib mesylate, LY2835219 (methanesulfonate), LY2835219, LY2835219 mesylate |
分子量 | 602.7 |
分子式 | C27H32F2N8·CH4O3S |
CAS No. | 1231930-82-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
H2O: 45mg/mL (74.7mM)
DMSO: 77 mg/mL (127.8 mM)
Ethanol: 23 mg/mL (38.2 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
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您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Abemaciclib methanesulfonate 1231930-82-7 Cell Cycle/Checkpoint CDK abemaciclib mesylate inhibit Inhibitor LY-2835219 Cyclin dependent kinase Abemaciclib LY 2835219 LY2835219 (methanesulfonate) LY2835219 LY2835219 mesylate inhibitor