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Anti-CDK4 Antibody (6D570)
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产品编号 TMAB-00406
Anti-CDK4 Antibody (6D570) 是一种 Mouse 抗体，靶向 CDK4。Anti-CDK4 Antibody (6D570) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
Anti-CDK4 Antibody (6D570)
Anti-CDK4 Antibody (6D570)
还可以产品编号 TMAB-00406
Anti-CDK4 Antibody (6D570) 是一种 Mouse 抗体，靶向 CDK4。Anti-CDK4 Antibody (6D570) 可用于 WB,IHC-P,IHC-Fr,ICC/IF,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,575 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-CDK4 Antibody (6D570) is a Mouse antibody targeting CDK4. Anti-CDK4 Antibody (6D570) can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF. |
| 克隆号 | 6D570 |
| 交叉反应 | Human,Mouse,Rat |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (human brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (human myocardium); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Cdk4) Monoclonal Antibody, Unconjugated (TMAB-00406) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 4. Sample: Lane 1: Embryo (Mouse) Lysate at 40 μg Lane 2: Placenta (Mouse) Lysate at 40 μg Lane 3: Heart (Mouse) Lysate at 40 μg Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 5: Heart (Rat) Lysate at 40 μg Lane 6: Cerebrum (Rat) Lysate at 40 μg Lane 7: Jurkat (Human) Cell Lysate at 30 μg Lane 8: Hela (Human) Cell Lysate at 30 μg Lane 9: MCF-7 (Human) Cell Lysate at 30 μg Lane 10: A431 (Human) Cell Lysate at 30 μg Primary: Anti-Cdk4 (TMAB-00406) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 34 kDa Observed band size: 32 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:50-200; IHC-Fr: 1:50-200; ICC/IF: 1:50-200; IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 亚细胞定位 | Cytoplasm. Nucleus. Membrane. Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus. |
| 构建方式 | Hybridoma Monoclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 免疫原 | KLH conjugated synthetic peptide: human Cdk4 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Cdk4,Cdks,Cdks,Cdks |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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