规格

价格

库存

数量

1 mg

¥ 161

现货

5 mg

¥ 369

现货

10 mg

¥ 533

现货

25 mg

¥ 987

现货

50 mg

¥ 1,650

现货

100 mg

¥ 2,560

现货

200 mg

¥ 3,790

现货

1 mL x 10 mM (in DMSO)

¥ 338

现货

生物活性

产品描述	PRIMA-1 (NSC-281668) is a mutant p53 reactivator. It induces apoptosis and inhibits growth of human tumors with mutant p53.
体外活性	PRIMA-1 is converted to compounds that form adducts with thiols in mutant p53. Modification of thiol groups in mutant p53 by PRIMA-1 conversion products is sufficient to restore its tumor suppressor activity.[2]. PRIMA-1 inhibits the growth of pancreatic cancer cell lines and induces cell cycle arrest and decreases DNA synthesis. It selectively induces apoptosis and cell death in mutant p53-expressing pancreatic cancer cells and also leads to activation of p53-dependent apoptotic pathways. PRIMA-1 enhances the cytotoxicity of chemotherapeutic agents active against mutant p53 pancreatic cancer cells[1]. PRIMA-1 has antileukemic properties in acute promyelocytic leukemia-derived NB4 cells. PRIMA-1-triggered apoptosis is in a dose-dependent and time-dependent manner as indicated by the MTT assay and annexin-V staining. Apoptosis induction by PRIMA-1 is associated with caspase-9, caspase-7 activation and PARP cleavage. PRIMA-1 does not show any significant apoptotic effect in normal human peripheral blood mononuclear cells[4].
体内活性	Intravenous (i.v.) injections of PRIMA-1 in mice does not cause any obvious changes in weight or behavior compared with untreated animals. PRIMA-1 has in vivo antitumor activity in this animal tumor model. It suppresses in vivo tumor growth in a mutant p53-dependent manner[3].
细胞实验	Cells are kept at a temperature of 37 °C, a minimum relative humidity of 95 %, and an atmosphere of 5 % CO2 in air. Cell viability is measured by MTT assay after treatment with PRIMA-1. Briefly, cells are seeded in each well of 96-well plates in 100 μl culture medium and incubated overnight at 37 °C in an atmosphere of 5 % CO2. The next day, the medium is removed and cells washed with PBS and treated with vehicle control(DMSO, dimethylsulfoxide) or different concentrations of PRIMA-1 for 12 to 48 h; the medium is replaced with MTT solution diluted in medium once the treatment is completed. The plates are further incubated at 37 °C under 5 % CO2 for 4 h and then left at room temperature until completely dry. DMSO was then added and the absorbance is read at 492 nm using a microplate enzyme-linked immunoassay reader (ELISA). The relative growth activity is determined as the percentage absorbance of treated cells compared to that of vehicle treated cells (control).(Only for Reference)
别名	PRIMA 1, NSC-281668, 2,2-Bis(hydroxymethyl)-3-quinuclidinone

化学信息

分子量	185.22
分子式	C₉H₁₅NO₃
CAS No.	5608-24-2
Smiles	OCC1(CO)N2CCC(CC2)C1=O
密度	1.32g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

Ethanol: 35 mg/mL (188.96 mM), Sonication is recommended.

H2O: 18.5 mg/mL (99.88 mM), Sonication is recommended.

DMSO: 50 mg/mL (269.95 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10.8 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.3990 mL	26.9949 mL	53.9898 mL	269.9492 mL
5 mM	1.0798 mL	5.3990 mL	10.7980 mL	53.9898 mL
10 mM	0.5399 mL	2.6995 mL	5.3990 mL	26.9949 mL
20 mM	0.2699 mL	1.3497 mL	2.6995 mL	13.4975 mL
50 mM	0.1080 mL	0.5399 mL	1.0798 mL	5.3990 mL