规格

价格

库存

数量

1 mg

¥ 163

现货

5 mg

¥ 357

现货

10 mg

¥ 648

现货

25 mg

¥ 998

现货

50 mg

¥ 1,330

现货

100 mg

¥ 2,030

现货

200 mg

¥ 3,050

待询

生物活性

产品描述	N-Formyl-Met-Leu-Phe (N-Formyl-MLF) (fMLF) is a synthetic peptide that acts as an agonist at formyl peptide receptors (FPR; Ki = 0.8 pM).
体外活性	N-Formyl-Met-Leu-Phe 受体与 Ni GTP 调节蛋白相关联，N-Formyl-Met-Leu-Phe 复合物在控制多个中性粒细胞功能方面具有重要作用[1]。
体内活性	N-Formyl-Met-Leu-Phe(fMLP)在斑马鱼和兔子中促进了骨骼形成，表明其在体内具有生理学相关性[2]。
动物实验	ebrafish (Danio rerio) embryos were collected from spontaneous spawnings reared at 28.5 ℃. Three days postfertilization (～1 day after hatching), feeding of the commercial powder for baby fish was started. Zebrafish embryos were stimulated with fMLP from 8 to 48 h postfertilization (hpf), washed, and raised at 28.5 ℃. At 5 days postfertilization (dpf), 20 zebrafish were stained with Alizarin Red for examination of the effect of fMLP on bone formation. Zebrafish were fixed in 4% paraformaldehyde for 12–18 h. Following fixation, fish were depigmented overnight in 1% KOH containing 3% H2O2 and immersed for 2 h in 0.5% KOH containing 0.05% Alizarin Red, pH 4.2. Alizarin-stained fish were then cleared again in 1% KOH and observed under a microscope[2].
别名	N-Formyl-MLF, fMLP

化学信息

分子量	437.55
分子式	C₂₁H₃₁N₃O₅S
CAS No.	59880-97-6
Smiles	CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1ccccc1)C(O)=O
密度	1.198 g/cm3 (Predicted)
Sequence	Formyl-Met-Leu-Phe
Sequence Short	Formyl-MLF

储存&溶解度

存储

keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

溶解度信息

DMSO: 120 mg/mL (274.25 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (7.54 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO