Name: TNFR2/CD120b/TNFR1B Protein, Human, Recombinant (His)
Brand: TargetMol
SKU: TMPY-01166
Price: 849 CNY
Availability: InStock
Rating: 5 (10 reviews)

规格

价格

库存

数量

5 μg

¥ 849

现货

10 μg

¥ 1,380

现货

20 μg

¥ 2,360

现货

50 μg

¥ 4,680

现货

100 μg

¥ 7,970

现货

200 μg

¥ 12,900

5日内发货

500 μg

¥ 25,300

5日内发货

1 mg

¥ 41,800

5日内发货

生物活性

产品描述	TNFR2/CD120b/TNFR1B Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.6 kDa and the accession number is P20333-1.
生物活性	1. Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-2 μg/ml in the presence of 0.25 ng/mL recombinant human TNF-α. 2. Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 2 μg/ml (100 μl/well) can bind Human TNFR2/TNFRSF1B His, the EC50 of Human TNFR2/TNFRSF1B His is 2.0-12.0 ng/mL.
研究背景	Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B), also known as Tumor necrosis factor receptor 2 (TNFR2) or CD120b antigen, is a member of the tumor necrosis factor receptor superfamily. TNFR2/CD120b/TNFRSF1B is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. TNFR2/CD120b/TNFRSF1B is not a major contributing factor to the genetic risk of type 2 diabetes, its associated peripheral neuropathy and hypertension and related metabolic traits in North Indians. Tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) has been reported to be associated with SLE risk in Japanese populations. TNFR2/CD120b/TNFRSF1B serves as a receptor with high affinity for TNFSF2 and approximately 5-fold lower affinity for homotrimeric TNFSF1. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P20333-1
蛋白构建	A DNA sequence encoding the extracellular domain (Met1-Asp257) of human TNFRII (NP_001057.1) was expressed with a C-terminal polyhistidine tag. Predicted N terminal: Leu 23
蛋白纯度	≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	tumor necrosis factor receptor superfamily, member 1b, TNF-R-II, TNFR80, TNF-R75, TNFR2, TNFR1B, TNFBR, TBPII, p75TNFR, p75, CD120b
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.