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TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated

产品编号 TMPY-07042TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated

TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is P19438-1.

TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated

TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated

产品编号 TMPY-07042TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated
TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is P19438-1.
规格价格库存数量
20 μg
¥ 3,820
期货
100 μg
¥ 8,830
期货
大包装 & 定制
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为什么重组蛋白的体积和质量与预期不符?
蛋白冻干粉由蛋白原液冻干而来,冻干粉质量不等同于蛋白质量。由于冻⼲产品的体积大⼩受多种因素影响,包括冻⼲前缓冲液的组分、盐离⼦浓度以及产品本身的浓度等。冻⼲粉可能看上去很多,也可能肉眼不易观察到,这些都是正常现象,请您放心使用。在收到产品后,照常复溶、使用或分装储存即可。
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产品信息

生物活性
Immobilized TNF-alpha Protein, Human, Recombinant (Cat#TMPY-00936) at 2 μg/mL (100 μl/well) can bind TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated (Cat#TMPY-07042), the EC50 is 2-6 ng/mL.
产品描述
TNFR1/CD120a/TNFRSF1A Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 Cells. The accession number is P19438-1.
种属
Human
表达系统
HEK293 Cells
标签C-Avi-His
蛋白编号P19438-1
别名
TNF-R-I,TNFR60,TNF-R55,TNFR55,TNFR1,TNF-R,TNFAR,TBP1,p60,p55-R,p55,FPF,CD120a
蛋白构建
A DNA sequence encoding the Human TNFRSF1A (NP_001056.1) (Met1-Thr211) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度
≥ 95% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC.
分子量24.5 kDa (predicted); 40.3 kDa (reducing contition)
内毒素< 1.0 EU per μg protein as determined by the LAL method.
缓冲液Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
复溶方法
Please refer to the lot-specific COA.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD120a (cluste of differentiation 120a), also known as TNFR1 / TNFRSF1A, is a member of CD family, tumor necrosis factor receptor superfamily. CD120a is one of the most primary receptors for the tumor necrosis factor-alpha. It has been shown to be localized to both plasma membrane lipid rafts and the trans golgi complex with the help of the death domain (DD). CD120a can activate the transcription factor NF-κB, mediate apoptosis, and regulate inflammation processes.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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请阅读 重组蛋白用户指南 了解更多具体信息.