UNC 569 hydrochloride inhibits Mer phosphorylation levels with IC50 values of 141 nM and 193 nM in human B-ALL 697 and Jurkat cell lines, respectively. UNC 569 hydrochloride (1 µM) treatment effectively inhibits the activation of Mer and downstream signaling, including the PI3K/AKT and MAPK/ERK pathways. In ALL cell lines, UNC 569 hydrochloride (0.4 -2 µM) induces apoptosis and increases the levels of cleaved Caspase 3 and cleaved PARP[1].

In Leukemic zebrafish, UNC 569 hydrochloride (4 µM) induces more than 50% reduction in tumor burden compared with vehicle- and mock-treated fish[1]. UNC 569 hydrochloride has low systemic clearance (19.5 mL/min/kg), high volume of distribution (Vss of 5.83 L/kg), and good oral bioavailability (57%)[2].