Anti-Phospho-AXL (Tyr698, 702, 703) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AXL (Tyr698, 702, 703). Anti-Phospho-AXL (Tyr698, 702, 703) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-AXl (Tyr698+Tyr702+Tyr703) Polyclonal Antibody, Unconjugated (TMAB-01394) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: A431 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140/120 kDa
Observed band size: 120 kDa
3. Sample: MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa
4. Sample: SW480 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human AXL around the phosphorylation site of Tyr698+Tyr702+Tyr703

Human

Kinases/phosphatases,Signal transducers,Signal transduction,Kinases/Phosphatases,Receptor Tyrosine Kinases,Tumor Associated