Anti-Phospho-AXL (Tyr698, 702, 703) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AXL (Tyr698, 702, 703). Anti-Phospho-AXL (Tyr698, 702, 703) Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P,WB.

IgG

Human (predicted:Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit)

1. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-phospho-AXl (Tyr698+Tyr702+Tyr703) Polyclonal Antibody, Unconjugated (TMAB-01394) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Sample:
Lane 1: A431 (Human) Cell Lysate at 30 μg
Lane 2: Hela (Human) Cell Lysate at 30 μg
Lane 3: 293T (Human) Cell Lysate at 30 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140/120 kDa
Observed band size: 120 kDa
3. Sample: MCF-7 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa
4. Sample: SW480 Cell (Human) Lysate at 40 μg
Primary: Anti-phospho-AXL (Tyr698+Tyr702+Tyr703) (TMAB-01394) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 120 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human AXL around the phosphorylation site of Tyr698+Tyr702+Tyr703

Human

AXL

Tyrosine-protein kinase receptor UFO

Kinases/phosphatases,Signal transducers,Signal transduction,Kinases/Phosphatases,Receptor Tyrosine Kinases,Tumor Associated

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol-3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. In case of filovirus infection, seems to function as a cell entry factor.