Anti-AXL Polyclonal Antibody is a Rabbit antibody targeting AXL. Anti-AXL Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse (predicted:Rat,Dog,Horse,Rabbit)

1. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (TMAB-00177) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AXL/UFO Polyclonal Antibody, Unconjugated (TMAB-00177) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Sample:
MKN45 (Human) Cell Lysate at 30 μg
SW480 (Human) Cell Lysate at 30 μg
FHc (Human) Cell Lysate at 30 μg
Primary: Anti-AXL (TMAB-00177) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140/120/98 kDa
Observed band size: 120 kDa
4. Sample:
Testis (Mouse) Lysate at 40 μg
Hela (Human) Cell Lysate at 30 μg
Primary: Anti-AXL (TMAB-00177) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 140/120/65 kDa
Observed band size: 120 kDa
5. Sample:
LOVO (Human) Cell Lysate at 40 μg
K562 (Human) Cell Lysate at 40 μg
Primary: Anti-AXL (TMAB-00177) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 95 kDa
Observed band size: 95 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human AXL

Human

Kinases/phosphatases,Signal transducers,Signal transduction,Kinases/Phosphatases,Receptor Tyrosine Kinases,Tumor Associated