规格

价格

库存

数量

100 mg

¥ 153

现货

500 mg

¥ 331

现货

1 g

¥ 415

现货

1 mL x 10 mM (in DMSO)

¥ 365

现货

生物活性

产品描述	TBHQ (tert-Butylhydroquinone) is an antioxidant that induces an antioxidant response through the redox-sensitive transcription factor Nrf2.
体外活性	HeLa细胞经t2,5-Di-tert-butylhydroquinone处理后，表现出对线粒体硫氧还蛋白-2(Trx2)的优先氧化，而细胞内谷胱甘肽和细胞质硫氧还蛋白-1未受影响。t2,5-Di-tert-butylhydroquinone处理促使Trx2过表达抑制了Nrf2的积累和活性[1]。在用t2,5-Di-tert-butylhydroquinone预处理Jurkat T细胞，并随之用抗CD3/抗CD28激活之前，能显著降低白介素-2(IL-2)在转录和蛋白水平上的产生。同样，CD25的表达也在经t2,5-Di-tert-butylhydroquinone预处理后减少，尽管此减少的程度较IL-2为轻[2]。
体内活性	TBHQ处理预防了TAC诱导的左室扩张和心脏功能障碍，并降低了心肌细胞凋亡的发生率。TBHQ诱导的Akt激活伴随着Bad、糖原合成酶激酶-3β（GSK-3β）和哺乳动物雷帕霉素靶蛋白（mTOR）的磷酸化增加。体内阻断Akt通路加速了心脏功能障碍，并消除了TBHQ的保护效果。TBHQ还减少了应激心肌中的反应性醛类产物和蛋白质氧化的产生[3]。
细胞实验	Jurkat T cells were cultured in 96-well plates (1×10^5 cells/well) and treated as described, and cell supernatants were harvested 24h after activation with anti-CD3/anti-CD28. IL-2 was quantified from cell supernatants by the use of a commercially available kit per the manufacturer's protocol. Relative light intensity was quantified at 450nm by Bio-Tek μQuant microplate reader. The IL-2 concentrations of each sample were then calculated using a line ar standard curve on Microsoft Excel [2].
动物实验	TBHQ powder was directly mixed with the animal food powder at a ratio of 1% (w/w) as described previously. The efficacy of this protocol for TBHQ treatment in vivo has also been validated by other groups. TBHQ treatment was continued for 4 weeks after the TAC surgery [3].
别名	特丁基对苯二酚, 叔丁基氢醌, tert-Butylhydroquinone

化学信息

分子量	166.22
分子式	C₁₀H₁₄O₂
CAS No.	1948-33-0
Smiles	C(C)(C)(C)C1=C(O)C=CC(O)=C1
密度	295 g/cm3

储存&溶解度

存储

溶解度信息

DMSO: 245 mg/mL (1473.95 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (30.08 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.0161 mL	30.0806 mL	60.1612 mL	300.8062 mL
5 mM	1.2032 mL	6.0161 mL	12.0322 mL	60.1612 mL
10 mM	0.6016 mL	3.0081 mL	6.0161 mL	30.0806 mL
20 mM	0.3008 mL	1.5040 mL	3.0081 mL	15.0403 mL
50 mM	0.1203 mL	0.6016 mL	1.2032 mL	6.0161 mL
100 mM	0.0602 mL	0.3008 mL	0.6016 mL	3.0081 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

TBHQ

TBHQ

产品介绍

计算器

体内实验配液计算器

剂量转换

引用文献

关键词

TBHQ

TBHQ

TBHQ 相关产品

产品介绍

计算器

体内实验配液计算器

剂量转换

引用文献

关键词