Anti-ERK1/2 Antibody (2U314) is a Mouse antibody targeting ERK1/2. Anti-ERK1/2 Antibody (2U314) can be used in WB,IHC-P,IHC-Fr,IF.

IgG

2U314

Mouse,Rat (predicted:Human)

1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1/2) Monoclonal Antibody, Unconjugated (TMAB-00635) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (TMAB-00635) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (ERK1+2) Polyclonal Antibody, Unconjugated (TMAB-00635) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.
4. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-ERK1/2 (TMAB-00635) at 1/1000 dilution
Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific
Predicted band size: 43 kDa
Observed band size: 44,42 kDa

WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Monoclonal

KLH conjugated synthetic peptide: human ERK1/2

Human

Tangles & Tau,MAPK Pathway,Cytoplasmic