规格

价格

库存

数量

1 mg

¥ 223

现货

5 mg

¥ 448

现货

10 mg

¥ 708

现货

25 mg

¥ 1,430

现货

50 mg

¥ 2,190

现货

100 mg

¥ 3,290

现货

200 mg

¥ 4,480

现货

1 mL x 10 mM (in DMSO)

¥ 488

现货

生物活性

产品描述	Epothilone A (Epo A) is a microtubule-stabilizing agent with EC0.01 of 2 μM.
靶点活性	Tubulin:2 μM(EC0.01)
体内活性	Epothilone A诱导微管蛋白聚合, 导致细胞周期在G2-M期停滞, 具有细胞毒性,也诱导细胞凋亡。Epothilone A有效抑制HCT116细胞增殖,IC50为4.4 nM。Epothilone A 对KB3-1, KBV-1, Hela和 Hs578T细胞具有细胞毒性,IC50为13 nM到160 nM。Epothilone A与紫杉醇竞争性与微管结合,IC50为2.3 μM。
激酶实验	Tubulin polymerization assay: Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone A is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope.
细胞实验	Cell lines: KB3-1,KBV-1,Hela,and Hs578T cells. Concentrations: 0–2 μM. Method: cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips.After 24 hours,cells are treated with Epothilone A and are scored at regular intervals.For the cytotoxicity analysis,cells are counted and scored as trypan blue positive or negative.
别名	埃博霉素 A, epothile, Epo A

化学信息

分子量	493.66
分子式	C₂₆H₃₉NO₆S
CAS No.	152044-53-6
Smiles	C[C@H]1CCC[C@H]2O[C@H]2C[C@H](OC(=O)C[C@H](O)C(C)(C)C(=O)[C@H](C)[C@H]1O)C(\C)=C\c1csc(C)n1
密度	1.143g/cm3

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 92 mg/mL (186.36 mM), Sonication is recommended.

DMSO: 92 mg/mL (186.36 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.68 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0257 mL	10.1284 mL	20.2569 mL	101.2843 mL
5 mM	0.4051 mL	2.0257 mL	4.0514 mL	20.2569 mL
10 mM	0.2026 mL	1.0128 mL	2.0257 mL	10.1284 mL
20 mM	0.1013 mL	0.5064 mL	1.0128 mL	5.0642 mL
50 mM	0.0405 mL	0.2026 mL	0.4051 mL	2.0257 mL
100 mM	0.0203 mL	0.1013 mL	0.2026 mL	1.0128 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Epothilone A

Epothilone A

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

Epothilone A

Epothilone A

Epothilone A 相关产品

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词