规格

价格

库存

数量

10 g

¥ 137

现货

1 mL x 10 mM (in DMSO)

¥ 228

现货

生物活性

产品描述	(±)-Carnitine chloride (Monocamin) is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine.
体外活性	L-Carnitine chloride的主要作用是通过内线粒体膜运送长链脂肪酸。在肉碱棕榈酰转移酶（CPT）-I激活后，L-Carnitine chloride和酰基辅酶A变为酰基-肉碱，该运输的酰基-肉碱在线粒体基质中由CPT-II转变为酰基辅酶A。L-Carnitine chloride处理增加了棕榈酰辅酶A诱导的线粒体呼吸，并在ADP存在下被加速。这种加速由L-Carnitine chloride处理以浓度依赖的方式诱导，并在5 mM L-Carnitine chloride时达到饱和[1]。L-Carnitine chloride预处理增强了Water2处理的HL7702细胞中Nrf2核内转位、DNA结合活性和血红素氧合酶-1（HO-1）表达。通过Akt介导的Nrf2信号通路激活，L-Carnitine chloride保护HL7702细胞免受Water2诱导的细胞损伤[2]。
体内活性	(±)-Carnitine chloride被发现能够下调泛素-蛋白酶体途径并增加动物模型中的IGF-1浓度。连续给予(±)-Carnitine chloride治疗2周的悬吊实验能够缓解腓肠肌重量和纤维尺寸的减少。此外，(±)-Carnitine chloride抑制atrogin-1 mRNA表达，该因子被认为在肌肉萎缩中发挥关键作用[3]。与(±)-Carnitine chloride的同时治疗减轻了与血浆TGF-β1水平降低相关的肾脏纤维化以及L-NAME组中报告的氧化和炎症状态，同时增加了PPAR-γ的表达[4]。
激酶实验	Mitochondria (0.6 mg protein/mL) are incubated in 2.5 mM Hepes (pH7.4) containing 225 mM mannitol, 75 mM sucrose and 100 μM ethylene glycol tetraacetic acid (EGTA) with or without 5 mM L-carnitine at 25°C. To measure oxygen uptake, 10 min after inorganic phosphate (Pi) 4 mM are added, the mitochondria are treated with palmitoyl-CoA (50 μM) and then ADP is added (200 μM). Oligomycin (5 μM) and rotenone (10 μM) are added 3-4 min after the ADP treatment. HPG (0-10 mM), which can specifically inhibit carnitine palmitoyl transferase (CPT)-I activity in the mitochondria, is added in the Hepes medium before incubation of the mitochondria[1].
别名	混旋肉碱盐酸盐, Monocamin, DL-Carnitine HCl, DL-Carnitine chloride, Bicarnesine

化学信息

分子量	197.66
分子式	C₇H₁₅NO₃·HCl
CAS No.	461-05-2
Smiles	[Cl-].C[N+](C)(C)CC(O)CC(O)=O
密度	no data available

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: 37 mg/mL (187.19 mM), Sonication is recommended.

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 45 mg/mL (227.66 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (10.12 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	5.0592 mL	25.2960 mL	50.5919 mL	252.9596 mL
5 mM	1.0118 mL	5.0592 mL	10.1184 mL	50.5919 mL
10 mM	0.5059 mL	2.5296 mL	5.0592 mL	25.2960 mL
20 mM	0.2530 mL	1.2648 mL	2.5296 mL	12.6480 mL
50 mM	0.1012 mL	0.5059 mL	1.0118 mL	5.0592 mL
100 mM	0.0506 mL	0.2530 mL	0.5059 mL	2.5296 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

(±)-Carnitine chloride

(±)-Carnitine chloride

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

(±)-Carnitine chloride

(±)-Carnitine chloride