购物车
  • TargetMol
    您的购物车当前为空
前往下单页继续购物
顶部相关产品产品介绍化学信息储存和溶解信息计算器

Ethidium bromide

一键复制产品信息
Rating icon 很棒

纯度: 99.14%

货号 T1220Cas号 1239-45-8

别名 溴化乙啶, Homidium bromide, EtBr, EB

Ethidium bromide (EB) 是一种可用作分子生物实验学中的荧光标记(核酸染色)，如琼脂糖凝胶电泳。Ethidium bromide 的λEx 为300-360 nm,λEm为590 nm。

Ethidium bromide
其他形式的 “Ethidium bromide”:
Endothelin B Receptor Protein, Human, Recombinant (His)
Endothelin B Receptor Protein, Human, Recombinant (mFc)

Ethidium bromide

一键复制产品信息
Rating icon 很棒

纯度: 99.14%

货号 T1220 别名 溴化乙啶, Homidium bromide, EtBr, EBCas号 1239-45-8

Ethidium bromide (EB) 是一种可用作分子生物实验学中的荧光标记(核酸染色)，如琼脂糖凝胶电泳。Ethidium bromide 的λEx 为300-360 nm,λEm为590 nm。

规格价格库存数量
25 mg
¥ 132
现货
50 mg
¥ 178
现货
100 mg
¥ 247
现货
500 mg
¥ 588
现货
1 g
¥ 863
现货
1 mL x 10 mM (in DMSO)
¥ 150
现货
库存状态实时更新，以官网显示为准，现货产品可直接加购物车下单
大包装 & 定制
加入购物车
TargetMol的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。
实验操作小课堂
常见问题解答
查看更多
选择批次:
纯度: 99.14%
联系我们 获取更多批次信息
资源下载:
COA LCMS HNMR产品操作手册

产品介绍

Ethidium bromide AI Summary
Ethidium bromide exhibits a broad spectrum of bioactivities, notably in DNA binding, antimicrobial function, and telomerase inhibition. The compound binds to calf thymus DNA and synthetic DNA and RNA duplexes, indicating a stabilizing and potentially intercalating effect on DNA structure. It shows significant increases in denaturation temperature (Tm) and high binding constants, demonstrating strong interaction with DNA. In DNA binding assays, Ethidium bromide has binding constants (K) ranging from 360,000 M^-1 to 1,200,000 M^-1, depending on the experimental conditions. The compound also displays affinity for DNA through fluorescence quenching assays (log Ks = 6.0) and surface plasmon resonance (Kd = 1200.0 nM). Ethidium bromide exhibits antibacterial activity against various strains of Escherichia coli and Staphylococcus aureus, with Minimum Inhibitory Concentration (MIC) values ranging from 2.0 to 2048.0 µg/mL. It shows enhanced antibacterial efficacy when combined with efflux pump inhibitors, suggesting potential utility in combating efflux-mediated resistance. The compound is effective against multidrug-resistant strains, including Escherichia coli harboring efflux pump genes and Staphylococcus aureus expressing NorA and other resistance genes. Additionally, Ethidium bromide displays inhibitory activity against telomerase in various human cell lines, including SGC7901, MGC803, and H9 cells, with IC50 values typically around 2000-3000 nM. It also shows potential anticancer properties by inhibiting the growth of human carcinoma cell lines and reducing mitochondrial DNA copy numbers, indicating cytotoxic effects. Importantly, Ethidium bromide demonstrates antiviral activity, notably against SARS-CoV-2-induced cytopathy in VERO-6 cells and Hepatitis C virus with varying levels of potency. Overall, Ethidium bromide presents multifaceted bioactivities with significant potential as an antimicrobial, antiviral, and anticancer agent, warranting further investigation and development..
Note: Summary generated by AI. Data source: ChEMBL
生物活性
产品描述
Ethidium bromide (EB) A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide. Ethidium bromide has a λEx of 300-360 nm and a λEm of 590 nm.
体外活性

使用方法
一、溶液配置：根据需要分离的带的大小配置合适浓度的琼脂糖凝胶（常用 0.5％至2.5％）。
二、操作步骤

  1. 精确称取一定量的琼脂糖粉与一定量的 1X TAE/TBE 混合。
  2. 用微波炉加热 1-3 分钟，直至琼脂糖完全溶解。
  3. 融化后的琼脂溶液摇晃混匀，倒入电泳槽中，等其凝固；
  4. 加入 Ethidium bromide （EtBr） 至最终浓度约为 0.2-0.5 μg/mL（原液通常为 10 mg/mL，需要 5 μL 原液/100 mL 凝胶）；
  5. Ethidium bromide 与 DNA 结合，在紫外线（UV）光下即可显现 DNA。
别名溴化乙啶, Homidium bromide, EtBr, EB
化学信息
分子量394.32
分子式C21H20BrN3
CAS No.1239-45-8
Smiles[Br-].CC[n+]1c2cc(N)ccc2c2ccc(N)cc2c1c1ccccc1
密度1.3739 g/cm3 (Estimated)
颜色Red
物理性状Solid
储存&溶解度
存储keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
溶解度信息
DMSO: 23.86 mg/mL (60.51 mM), Sonication is recommended.
H2O: 1 mg/mL (2.54 mM), Sonication is recommended.
溶液配制表
H2O/DMSO
1mg5mg10mg50mg
1 mM2.5360 mL12.6801 mL25.3601 mL126.8006 mL
DMSO
1mg5mg10mg50mg
5 mM0.5072 mL2.5360 mL5.0720 mL25.3601 mL
10 mM0.2536 mL1.2680 mL2.5360 mL12.6801 mL
20 mM0.1268 mL0.6340 mL1.2680 mL6.3400 mL
50 mM0.0507 mL0.2536 mL0.5072 mL2.5360 mL

计算器

  • 摩尔浓度 计算器
  • 稀释 计算器
  • 配液 计算器
  • 分子量 计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：
TargetMol | Animal experiments 比如您的给药剂量是10 mg/kg，每只动物体重20g，给药体积100 μL， TargetMol | Animal experiments 一共给药动物10只，您使用的配方为5%TargetMol | reagent DMSO + 30%PEG300 + 5%Tween 80 + 60%Saline/PBS/ddH2O， 那么您的工作液浓度为2mg/mL。
母液配置方法: 2 mg 药物溶于 50 μLDMSOTargetMol | reagent （ 母液浓度为 40 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。
体内配方的制备方法: 取 50μLDMSOTargetMol | reagent 母液，添加 300 μLPEG300TargetMol | reagent 混匀澄清，再加 50μLTween80, 混匀澄清，再加 600μLSaline/PBS/ddH2OTargetMol | reagent 混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

方案所需的各类助溶剂如： DMSO ， PEG300 / PEG400 ， Tween 80 ， SBE-β-CD ， 玉米油 等， 均可在TargetMol网站点击购买。
1 请输入动物实验的基本信息
mg/kg
g
μL
2 请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。
% DMSO
%
% Tween 80
% Saline/PBS/ddH2O

剂量转换

对于不同动物的给药剂量换算，您也可以参考 更多

关键词

nAChRInhibitorinhibitHomidium BromideHomidiumEthidium BromideEthidium
Related Tags: buy Ethidium bromide | purchase Ethidium bromide | Ethidium bromide cost | order Ethidium bromide | Ethidium bromide chemical structure | Ethidium bromide in vitro | Ethidium bromide formula | Ethidium bromide molecular weight