Deoxycholic acid (DCA) is a natural product and a TGR5 receptor agonist with cell permeability and oral activity. As a metabolite of intestinal flora, this compound is widely used in research on bile acid signal transduction, glucose and lipid metabolism, and anti-inflammatory mechanisms, and has shown therapeutic potential in diabetes and liver disease models.

CSNK1E:7.22 μM (EC50), Nicastrin:6.03 μM (EC50), FATP5:0.19 μM, NTCP:2.14 µM, FATP2:5 μM

Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining. (Only for Reference)

脱氧胆酸, 去氧胆酸, Desoxycholic acid, Deoxycholate, DCA, Cholorebic, Cholerebic, Cholanoic Acid

DMSO: 145 mg/mL (369.36 mM), Sonication is recommended.

Ethanol: 56 mg/mL (142.65 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)