规格

价格

库存

数量

50 mg

¥ 277

现货

100 mg

¥ 460

现货

200 mg

¥ 840

现货

500 mg

¥ 1,421

现货

1 mL x 10 mM (in DMSO)

¥ 426

现货

生物活性

产品描述	AEBSF hydrochloride (Pefabloc SC) is an irreversible inhibitor of serine proteases, such as chymotrypsin, kallikrein, thrombin, plasmin, and trypsin.
体外活性	在蟑螂诱发的小鼠过敏模型中,AEBSF能够减少其呼吸道反应和潜在的炎症.在小鼠中注射致死量的弓形虫,每天腹腔注射76.8 mg/kg AEBSF,能够延长其生存期.
体内活性	AEBSF是一种丝氨酸蛋白酶抑制剂,它能够抑制巨噬细胞裂解白血病细胞,并且不会抑制巨噬细胞分泌TNF-α和IL-1β。在5种不同人细胞系中,AEBSF通过抑制神经和非神经中的β分泌,从而抑制组成Aβ的产物。AEBSF能够扰乱子宫内膜中囊胚的生长,还能够改变蛋白分泌机制从而抑制在人脐静脉内皮细胞中HeLa细胞的粘附。
细胞实验	The HeLa cells suspended in RPMI-1640 media containing 10% FCS are plated into each well of a 96-well microplate (5×103 cells/200 μL/well). After incubation for 24 h at 37°C, cells are treated with different doses of AEBSF (0, 25, 50, 100 μg/mL) for 48 h. Then, 20 μL fresh 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-diphenytetrazoliumromide (MTT) reagent (5 μg/μL) is added into each well, and cells are cultured at 37°C in 5% CO2 for another 4 h. The media are discarded carefully, and 150 μL DMSO is added. Absorbance is read on a microplate reader at dual wavelengths of 540 and 620 nm.
别名	Pefabloc SC, AEBSF HCl

化学信息

分子量	239.69
分子式	C₈H₁₁ClFNO₂S
CAS No.	30827-99-7
Smiles	Cl.NCCc1ccc(cc1)S(F)(=O)=O
密度	no data available

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

实际储存温度请以COA为准

溶解度信息

H2O: 24 mg/mL (100.13 mM), Sonication is recommended.

DMSO: 247 mg/mL (1030.5 mM), Sonication is recommended.

体内实验配方

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (20.86 mM), Sonication is recommended.

请按顺序添加溶剂，在添加下一种溶剂之前，尽可能使溶液澄清。如有必要，可通过加热、超声、涡旋处理进行溶解。工作液建议现配现用。以上配方仅供参考，体内配方并不是绝对的，请根据不同情况进行调整。

溶液配制表

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1721 mL	20.8603 mL	41.7206 mL	208.6028 mL
5 mM	0.8344 mL	4.1721 mL	8.3441 mL	41.7206 mL
10 mM	0.4172 mL	2.0860 mL	4.1721 mL	20.8603 mL
20 mM	0.2086 mL	1.0430 mL	2.0860 mL	10.4301 mL
50 mM	0.0834 mL	0.4172 mL	0.8344 mL	4.1721 mL
100 mM	0.0417 mL	0.2086 mL	0.4172 mL	2.0860 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

AEBSF hydrochloride

AEBSF hydrochloride

产品介绍

引用文献

计算器

体内实验配液计算器

剂量转换

关键词

AEBSF hydrochloride

AEBSF hydrochloride