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M344

M344

产品编号 T2157   CAS 251456-60-7
别名: MS 344, Histone Deacetylase Inhibitor III

M344 (Histone Deacetylase Inhibitor III) 是一种组蛋白去乙酰化酶抑制剂,IC50为 100 nM。

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M344 Chemical Structure
M344, CAS 251456-60-7
规格 价格/CNY 货期 数量
1 mg ¥ 272 现货
5 mg ¥ 618 现货
10 mg ¥ 981 现货
25 mg ¥ 1,630 现货
50 mg ¥ 2,430 现货
100 mg ¥ 3,630 现货
1 mL * 10 mM (in DMSO) ¥ 618 现货
产品目录号及名称: M344 (T2157)
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生物活性
化学信息
存储 & 溶解度
参考文献
产品描述 M344 (Histone Deacetylase Inhibitor III) is a potent HDAC inhibitor with IC50 of 100 nM and able to induce cell differentiation.
靶点活性 HDAC:100 nM
体内活性 在体外,M344显示出对子宫内膜癌细胞系Ishikawa和卵巢癌细胞系SK-OV-3的显著抗增殖活性,EC50分别为2.3 μM和5.1 μM。而正常的人子宫内膜上皮细胞对M344几乎不敏感。高于10 μM浓度时的M344具有毒性,而最多只有20%的存活细胞群被诱导分化。此外,M344也会导致细胞周期中S期细胞比例减少,G0/G1期细胞比例增加,诱导细胞凋亡,并降低线粒体的跨膜电位。
激酶实验 Enzyme Inhibition : Radioactively labeled chicken core histones are used as the enzyme substrate. The enzyme liberated tritiated acetic acid from the substrate which is quantitated by scintillation counting. IC50 values are results of triple determinations. 50 μL of maize enzyme (at 30 °C) is incubated (30 minutes) with 10 μL of total [3H]acetate-prelabeled chicken reticulocyte histones (1 mg/mL). Reaction is stopped by addition of 36 μL of 1 M HCl/0.4 M acetate and 800 μL of ethyl acetate. After centrifugation (10000 g, 5 minutes) an aliquot of 600 μL of the upper phase is counted for radioactivity in 3 mL of liquid scintillation cocktail. M344 is tested in a starting concentration of 40 μM, and active substances are diluted further.
细胞实验 MEL DS19 cells (murine erythroleukemia cells) are maintained in D-MEM containing 100 units/mL penicillin G sodium and 100 μg/mL streptomycin sulfate supplemented with 10% fetal bovine serum at 37 °C in a 5% CO2 atmosphere. To test M344 for potential to induce cell differentiation, log-phase cells with a population doubling time of 11−13 hours are used. Serial dilutions of M344 are prepared in 24-well plates using 1 mL of D-MEM/well. If M344 are dissolved in DMSO, control wells contains the same amount of solvent (generally 2 μL/mL of medium). Subsequently, the cell suspension is added to the wells. After 72 hours the experiment is evaluated. Cell numbers are counted using a Casy 1 TTC flow cytometer. The proliferation of treated cells is expressed as percent proliferation in comparison with the solvent control. Differentiated MEL cells accumulate hemoglobin. Therefore, the induction of cell differentiation is determined by benzidine staining according to the literature. To 100 μL of cell suspension is added 10 μL of a 0.4% solution of benzidine in 12% acetic acid containing 2% Water2. Within 5 minutes hemoglobin-containing cells stains blue. Benzidine-positive and -negative cells are counted under the microscope in a hemocytometer, and the percentage of positive cells is calculated. M344 is first tested at 10 μM and 50 μM final concentration. According to activity/toxicity profile, a range of concentrations is chosen for a dose−response analysis. (Only for Reference)
别名 MS 344, Histone Deacetylase Inhibitor III
分子量 307.39
分子式 C16H25N3O3
CAS No. 251456-60-7

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

Ethanol: 4 mg/mL (13.01 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 62 mg/mL (201.69 mM)

溶液配制表

可选溶剂 浓度 体积 质量 1 mg 5 mg 10 mg 25 mg
Ethanol / DMSO 1 mM 3.2532 mL 16.266 mL 32.532 mL 81.3299 mL
5 mM 0.6506 mL 3.2532 mL 6.5064 mL 16.266 mL
10 mM 0.3253 mL 1.6266 mL 3.2532 mL 8.133 mL
DMSO 20 mM 0.1627 mL 0.8133 mL 1.6266 mL 4.0665 mL
50 mM 0.0651 mL 0.3253 mL 0.6506 mL 1.6266 mL
100 mM 0.0325 mL 0.1627 mL 0.3253 mL 0.8133 mL

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器
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输入分子式,点击计算,可计算出产品的分子量。

参考文献

1. Jung M, et al. J Med Chem. 1999, 42(22), 4669-4679. 2. Takai N, et al. Gynecol Oncol. 2006, 101(1), 108-113. 3. Furchert SE, et al. Int J Cancer. 2007, 120(8), 1787-1794. 4. St Germain C, et al. Cancer Cell Int. 2010,10, 32.
Suberoyl bis-hydroxamic acid MC1568 GK718 Dacinostat Domatinostat FT895 Scriptaid HDAC1/CDK7-IN-1

相关化合物库

该产品包含在如下化合物库中:
抗衰老化合物库 已知活性化合物库 组蛋白修饰化合物库 抗胰腺癌化合物库 HIF-1化合物库 NO PAINS 化合物库 DNA 损伤和修复分子库 免疫/炎症分子化合物库 抗癌化合物库 表观遗传库

剂量换算

对于不同动物的给药剂量换算,您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。

体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。

第一步:请输入动物实验的基本信息
剂量
mg/kg
每只动物体重
g
给药体积
μL
动物数量
第二步:请输入动物体内配方组成,不同的产品配方组成不同,如有配方需求,可先联系我们提供正确的体内配方。
% DMSO
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% Tween 80
% ddH2O
计算 重置

技术支持

您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。

Keywords

M344 251456-60-7 Chromatin/Epigenetic DNA Damage/DNA Repair HDAC inhibit Inhibitor MS-344 D-237 MS 344 Histone Deacetylase Inhibitor III D237 M-344 D 237 M 344 Histone deacetylases MS344 inhibitor

 

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