keep away from moisture|Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Aprotinin (Traskolan) 是分离自牛肺的一种广谱丝氨酸蛋白酶抑制剂,可抑制多种不同酯酶和蛋白酶的活性。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 410 | 现货 | ||
10 mg | ¥ 492 | 现货 | ||
25 mg | ¥ 1,060 | 现货 | ||
50 mg | ¥ 1,880 | 现货 | ||
100 mg | ¥ 2,890 | 现货 | ||
200 mg | ¥ 3,590 | 现货 | ||
500 mg | ¥ 5,790 | 现货 |
产品描述 | Aprotinin (Traskolan) a broad-spectrum serine protease inhibitor, inhibiting the activity of a number of different esterases and proteases, including trypsin, chymotrypsin, kallikrein, plasmin, tissue plasminogen activator, and tissue and leukocytic proteinases. |
靶点活性 | Trypsinogen:2 μM(Kd), Chymotrypsin:9.5 nM(Kd), Kallikrein:0.8 nM(Kd), Trypsin:0.06 pM(Kd) |
体外活性 | Aprotinin是一种抗纤溶分子,能够抑制胰蛋白酶和相关的蛋白水解酶。在细胞生物学中,aprotinin被用作酶抑制剂,以防止在细胞和组织的裂解或均质化过程中蛋白质降解。在aprotinin的存在下,纤溶活性被浓度依赖性地抑制,且凝血时间得以延长。Aprotinin是体外接触(内源性)凝血途径的有效抑制剂[2]。 |
体内活性 | Aprotinin 在体外可抑制凝块溶解,同时在体内可延长大鼠尾部出血时间及人类血浆的凝血时间。在大鼠动静脉分流模型中,aprotinin 能减轻血栓重量[2]。 |
激酶实验 | Substrates and kinases are diluted in 50?mM Tris/HCl (pH?7.5), 0.1% 2-mercaptoethanol, 0.1?mM EGTA and 10?mM magnesium acetate. Reactions are initiated with [γ-32P]ATP (2500 c.p.m./pmol) to a final concentration of 0.1?mM and terminated after 15?min at 30°C by the addition of SDS and EDTA (pH?7.0) to final concentrations of 1.0% (w/v) and 20?mM respectively. After heating for 5?min at 100°C and separation by SDS/PAGE, the phosphorylated proteins are detected by autoradiography. |
细胞实验 | Mouse G8-1 myoblasts are plated DMEM + 20% FBS (maintenance medium), in which they remain undifferentiated. When cells reach approximately 40-50% confluence, different protease inhibitors are added to the culture media and cells are incubated overnight. Cells are then switched to differentiation-promoting media (DMEM + 10% horse serum ± protease inhibitor) and incubated for 7 days. (Only for Reference) |
别名 | 抑酶肽, 抑肽酶, Bovine Pancreatic Trypsin Inhibitor, Traskolan, Antilysin |
化合物与蛋白结合的复合物 |
Crystal structure of aprotinin in complex with sucrose octasulfate: unusual interactions and implication for heparin binding |
分子量 | 6511.51 |
分子式 | C284H432N84O79S7 |
CAS No. | 9087-70-1 |
keep away from moisture|Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 100 mg/mL (15.36 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O | 1 mM | 0.1536 mL | 0.7679 mL | 1.5357 mL | 3.8394 mL |
5 mM | 0.0307 mL | 0.1536 mL | 0.3071 mL | 0.7679 mL | |
10 mM | 0.0154 mL | 0.0768 mL | 0.1536 mL | 0.3839 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Aprotinin 9087-70-1 Microbiology/Virology Others Proteases/Proteasome Ubiquitination Proteasome Influenza Virus Serine Protease Ser/Thr Protease Inhibitor 抑酶肽 抑肽酶 Serine proteases Serine endopeptidases Bovine Pancreatic Trypsin Inhibitor inhibit Traskolan Threonine proteases Antilysin inhibitor